TY - JOUR
T1 - β-catenin and TCF mediate cell positioning in the intestinal epithelium by controlling the expression of EphB/EphrinB
AU - Batlle, Eduard
AU - Henderson, Jeffrey T.
AU - Beghtel, Harry
AU - Van den Born, Maaike M.W.
AU - Sancho, Elena
AU - Huls, Gerwin
AU - Meeldijk, Jan
AU - Robertson, Jennifer
AU - Van de Wetering, Marc
AU - Pawson, Tony
AU - Clevers, Hans
N1 - Funding Information:
We thank Prof. R. Klein, Prof. K. Artories, Prof. L.M. Matrisian, Prof. J.I Gordon, Dr. J. Fawcett, Dr. C. Bolós, Prof. D. Louvard, Prof. A.B. Leiter, and Dr. S. Robine for valuable reagents. E.B. and E.S. hold Marie Curie Fellowships. This research was supported by a grant from the Canadian Institute for Health Research (CIHR) to T.P. and J.T.H. H.C. is supported by grants from the Netherlands Cancer Foundation. T.P. is a Distinguished Scientist of the CIHR.
PY - 2002/10/18
Y1 - 2002/10/18
N2 - In the small intestine, the progeny of stem cells migrate in precise patterns. Absorptive, enteroendocrine, and goblet cells migrate toward the villus while Paneth cells occupy the bottom of the crypts. We show here that β-catenin and TCF inversely control the expression of the EphB2/EphB3 receptors and their ligand ephrin-B1 in colorectal cancer and along the crypt-villus axis. Disruption of EphB2 and EphB3 genes reveals that their gene products restrict cell intermingling and allocate cell populations within the intestinal epithelium. In EphB2/EphB3 null mice, the proliferative and differentiated populations intermingle. In adult EphB3-/- mice, Paneth cells do not follow their downward migratory path, but scatter along crypt and villus. We conclude that in the intestinal epithelium β-catenin and TCF couple proliferation and differentiation to the sorting of cell populations through the EphB/ephrin-B system.
AB - In the small intestine, the progeny of stem cells migrate in precise patterns. Absorptive, enteroendocrine, and goblet cells migrate toward the villus while Paneth cells occupy the bottom of the crypts. We show here that β-catenin and TCF inversely control the expression of the EphB2/EphB3 receptors and their ligand ephrin-B1 in colorectal cancer and along the crypt-villus axis. Disruption of EphB2 and EphB3 genes reveals that their gene products restrict cell intermingling and allocate cell populations within the intestinal epithelium. In EphB2/EphB3 null mice, the proliferative and differentiated populations intermingle. In adult EphB3-/- mice, Paneth cells do not follow their downward migratory path, but scatter along crypt and villus. We conclude that in the intestinal epithelium β-catenin and TCF couple proliferation and differentiation to the sorting of cell populations through the EphB/ephrin-B system.
UR - https://www.scopus.com/pages/publications/18644376279
U2 - 10.1016/S0092-8674(02)01015-2
DO - 10.1016/S0092-8674(02)01015-2
M3 - Article
C2 - 12408869
AN - SCOPUS:18644376279
SN - 0092-8674
VL - 111
SP - 251
EP - 263
JO - Cell
JF - Cell
IS - 2
ER -