TY - JOUR
T1 - A central role for P48/45 in malaria parasite male gamete fertility
AU - Van Dijk, Melissa R.
AU - Janse, Chris J.
AU - Thompson, Joanne
AU - Waters, Andrew P.
AU - Braks, Joanna A.M.
AU - Dodemont, Huub J.
AU - Stunnenberg, Henk G.
AU - Van Gemert, Geert Jan
AU - Sauerwein, Robert W.
AU - Eling, Wijnand
N1 - Funding Information:
We would like to thank Jai Ramesar, Auke van Wigcheren, Marga van der Vegte-Bolmer, Ton Lensen, and Jo Hooghof for excellent technical assistance; Annemarie van der Wel and Clemens Kocken for great help with the fertilization assays; and Prof. R. E. Sinden for the gift of antibodies. Financial support was from the QLRT-KA2 program of the DGXII Department of the European Commission (Contract Number QLK2-CT-1999-00753), the Medical Sciences Foundation (901-15-079), which is subsidized by the Netherlands Organization for Scientific Research, and DGIS/SO grant N1002701. Sequence data for P. falciparum chromosome 13 was obtained from The Sanger Centre website at http://www.sanger.ac.uk/Projects/P_falciparum/ . Sequencing of P. falciparum chromosome 13 was accomplished as part of the Malaria Genome Project with support by The Wellcome Trust. Preliminary sequence data for P. falciparum chromosomes 10 and 11 were obtained from The Institute for Genomic Research website (www.tigr.org). Sequencing of chromosomes 10 and 11 was part of the International Malaria Genome Sequencing Project and was supported by an award from the National Institute of Allergy and Infectious Diseases, National Institutes of Health. Sequence data for P. berghei were obtained from the University of Florida Gene Sequence Tag Project Website at: http://parasite.vetmed.ufl.edu . Funding was provided by the National Institute of Allergy and Infectious Diseases.
Funding Information:
A contig of the Pfs48/45 and Pf47 locus was assembled from BLAST analysis of sequence data for P. falciparum chromosome 13 ( http://www.sanger.ac.uk/Projects/P_falciparum/ ). Sequencing of P. falciparum chromosome 13 was accomplished as part of the Malaria Genome Project with support by The Wellcome Trust. Preliminary sequence data for P. falciparum chromosomes 10 and 11 were obtained from The Institute for Genomic Research ( www.tigr.org ). For P. falciparum, primer pair irR (nt 14–25) and irF (nt 1206–1227) of pfs48/45 and pf47, respectively, and for P. berghei, primer pairs #486 and #651 corresponding to nt 1210–1233 of pbs47 were used to amplify the intergenic region by PCR.
PY - 2001/1/12
Y1 - 2001/1/12
N2 - Fertilization and zygote development are obligate features of the malaria parasite life cycle and occur during parasite transmission to mosquitoes. The surface protein PFS48/45 is expressed by male and female gametes of Plasmodium falciparum and PFS48/45 antibodies prevent zygote development and transmission. Here, gene disruption was used to show that Pfs48/45 and the ortholog Pbs48/45 from a rodent malaria parasite P. berghei play a conserved and important role in fertilization. p48/45- parasites had a reduced capacity to produce oocysts in mosquitoes due to greatly reduced zygote formation. Unexpectedly, only male gamete fertility of p48/45- parasites was affected, failing to penetrate otherwise fertile female gametes. P48/45 is shown to be a surface protein of malaria parasites with a demonstrable role in fertilization.
AB - Fertilization and zygote development are obligate features of the malaria parasite life cycle and occur during parasite transmission to mosquitoes. The surface protein PFS48/45 is expressed by male and female gametes of Plasmodium falciparum and PFS48/45 antibodies prevent zygote development and transmission. Here, gene disruption was used to show that Pfs48/45 and the ortholog Pbs48/45 from a rodent malaria parasite P. berghei play a conserved and important role in fertilization. p48/45- parasites had a reduced capacity to produce oocysts in mosquitoes due to greatly reduced zygote formation. Unexpectedly, only male gamete fertility of p48/45- parasites was affected, failing to penetrate otherwise fertile female gametes. P48/45 is shown to be a surface protein of malaria parasites with a demonstrable role in fertilization.
UR - https://www.scopus.com/pages/publications/17744374469
U2 - 10.1016/S0092-8674(01)00199-4
DO - 10.1016/S0092-8674(01)00199-4
M3 - Article
C2 - 11163248
AN - SCOPUS:17744374469
SN - 0092-8674
VL - 104
SP - 153
EP - 164
JO - Cell
JF - Cell
IS - 1
ER -