TY - JOUR
T1 - A critical role for the Wnt Effector Tcf4 in adult intestinal homeostatic self-renewal
AU - van Es, Johan H.
AU - Haegebarth, Andrea
AU - Kujala, Pekka
AU - Itzkovitz, Shalev
AU - Koo, Bon Kyoung
AU - Boj, Sylvia F.
AU - Korving, Jeroen
AU - van den Born, Maaike
AU - van Oudenaarden, Alexander
AU - Robin, Sylvie
AU - Clevers, Hans
PY - 2012/5
Y1 - 2012/5
N2 - Throughout life, intestinal Lgr5+ stem cells give rise to proliferating transient amplifying cells in crypts, which subsequently differentiate into one of the five main cell types and migrate along the crypt-villus axis. These dynamic processes are coordinated by a relatively small number of evolutionarily conserved signaling pathways, which includes the Wnt signaling pathway. The DNA-binding proteins of the T-cell factor family, Tcf1/Tcf7, Lef, Tcf3/Tcf7l1, and Tcf4/Tcf7l2, constitute the downstream effectors of the Wnt signaling pathway. While Tcf4 is the major member active during embryogenesis, the role of these Wnt effectors in the homeostasis of the adult mouse intestinal epithelium is unresolved. Using Tcf1-/-, Tcf3flox, and novel Tcf4flox mice, we demonstrate an essential role for Tcf4 during homeostasis of the adult mouse intestine.
AB - Throughout life, intestinal Lgr5+ stem cells give rise to proliferating transient amplifying cells in crypts, which subsequently differentiate into one of the five main cell types and migrate along the crypt-villus axis. These dynamic processes are coordinated by a relatively small number of evolutionarily conserved signaling pathways, which includes the Wnt signaling pathway. The DNA-binding proteins of the T-cell factor family, Tcf1/Tcf7, Lef, Tcf3/Tcf7l1, and Tcf4/Tcf7l2, constitute the downstream effectors of the Wnt signaling pathway. While Tcf4 is the major member active during embryogenesis, the role of these Wnt effectors in the homeostasis of the adult mouse intestinal epithelium is unresolved. Using Tcf1-/-, Tcf3flox, and novel Tcf4flox mice, we demonstrate an essential role for Tcf4 during homeostasis of the adult mouse intestine.
UR - http://www.scopus.com/inward/record.url?scp=84861372662&partnerID=8YFLogxK
U2 - 10.1128/MCB.06288-11
DO - 10.1128/MCB.06288-11
M3 - Article
C2 - 22393260
AN - SCOPUS:84861372662
SN - 0270-7306
VL - 32
SP - 1918
EP - 1927
JO - Molecular and Cellular Biology
JF - Molecular and Cellular Biology
IS - 10
ER -