A novel and fast normalization method for high-density arrays

Maarten van Iterson, Floor A M Duijkers, Jules P P Meijerink, Pieter Admiraal, Gert-Jan B van Ommen, Judith M Boer, Max M van Noesel, Renee X Menezes

Research output: Contribution to journalArticlepeer-review

Abstract

BACKGROUND: Among the most commonly applied microarray normalization methods are intensity-dependent normalization methods such as lowess or loess algorithms. Their computational complexity makes them slow and thus less suitable for normalization of large datasets. Current implementations try to circumvent this problem by using a random subset of the data for normalization, but the impact of this modification has not been previously assessed. We developed a novel intensity-dependent normalization method for microarrays that is fast, simple and can include weighing of observations.

RESULTS: Our normalization method is based on the P-spline scatterplot smoother using all data points for normalization. We show that using a random subset of the data for normalization should be avoided as unstable results can be produced. However, in certain cases normalization based on an invariant subset is desirable, for example, when groups of samples before and after intervention are compared. We show in the context of DNA methylation arrays that a constant weighted P-spline normalization yields a more reliable normalization curve than the one obtained by normalization on the invariant subset only.

CONCLUSIONS: Our novel intensity-dependent normalization method is simpler and faster than current loess algorithms, and can be applied to one- and two-colour array data, similar to normalization based on loess.

AVAILABILITY: An implementation of the method is currently available as an R package called TurboNorm from www.bioconductor.org.

Original languageEnglish
JournalStatistical applications in genetics and molecular biology
Volume11
Issue number4
DOIs
Publication statusPublished - 12 Jul 2012
Externally publishedYes

Keywords

  • Computational Biology/methods
  • High-Throughput Screening Assays/methods
  • Humans
  • Microarray Analysis/methods
  • Random Allocation
  • Reference Standards
  • Software
  • Time Factors
  • Validation Studies as Topic

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