Affitins: Ribosome Display for Selection of Aho7c-Based Affinity Proteins

Valentina Kalichuk, Stanimir Kambarev, Ghislaine Béhar, Benjamin Chalopin, Axelle Renodon-Cornière, Barbara Mouratou, Frédéric Pecorari

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

Abstract

Engineered protein scaffolds have made a tremendous contribution to the panel of affinity tools owing to their favorable biophysical properties that make them useful for many applications. In 2007, our group paved the way for using archaeal Sul7d proteins for the design of artificial affinity ligands, so-called Affitins. For many years, Sac7d and Sso7d have been used as molecular basis to obtain binders for various targets. Recently, we characterized their old gifted protein family and identified Aho7c, originating from Acidianus hospitalis, as the shortest member (60 amino-acids) with impressive stability (96.5 °C, pH 0–12). Here, we describe the construction of Aho7c combinatorial libraries and their use for selection of binders by ribosome display.
Original languageEnglish
Title of host publicationMethods in Molecular Biology
Pages19-41
Number of pages23
DOIs
Publication statusPublished - 2020

Publication series

NameMethods in Molecular Biology
Volume2070

Keywords

  • Affitin
  • Aho7c
  • In vitro selection
  • Ribosome display
  • Sac7d
  • Sso7d
  • Sul7d

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