TY - JOUR
T1 - CDK2AP1/DOC-1 is a bona fide subunit of the Mi-2/NuRD complex
AU - Spruijt, Cornelia G.
AU - Bartels, Stefanie J.J.
AU - Brinkman, Arie B.
AU - Tjeertes, Jorrit V.
AU - Poser, Ina
AU - Stunnenberg, Hendrik G.
AU - Vermeulen, Michiel
PY - 2010/9
Y1 - 2010/9
N2 - The Mi-2/NuRD (NUcleosome Remodeling and histone Deacetylase) chromatin remodeling complex is a large heterogeneous multiprotein complex associated with transcriptional repression. Here we apply a SILAC based quantitative proteomics approach to show that all known Mi-2/NuRD complex subunits co-purify with Cyclin Dependent Kinase 2 Associated Protein1 (CDK2AP1), also known as Deleted in Oral Cancer 1 (DOC-1). DOC-1 displays in vitro binding affinity for methylated DNA as part of the meCpG binding MBD2/NuRD complex. In luciferase reporter assays, DOC-1 is a potent repressor of transcription. Finally, immunofluorescence experiments reveal co-localization between MBD2 and DOC-1 in mouse NIH-3T3 nuclei. Collectively, these results indicate that DOC-1 is a bona fide subunit of the Mi-2/NuRD chromatin remodeling complex.
AB - The Mi-2/NuRD (NUcleosome Remodeling and histone Deacetylase) chromatin remodeling complex is a large heterogeneous multiprotein complex associated with transcriptional repression. Here we apply a SILAC based quantitative proteomics approach to show that all known Mi-2/NuRD complex subunits co-purify with Cyclin Dependent Kinase 2 Associated Protein1 (CDK2AP1), also known as Deleted in Oral Cancer 1 (DOC-1). DOC-1 displays in vitro binding affinity for methylated DNA as part of the meCpG binding MBD2/NuRD complex. In luciferase reporter assays, DOC-1 is a potent repressor of transcription. Finally, immunofluorescence experiments reveal co-localization between MBD2 and DOC-1 in mouse NIH-3T3 nuclei. Collectively, these results indicate that DOC-1 is a bona fide subunit of the Mi-2/NuRD chromatin remodeling complex.
UR - http://www.scopus.com/inward/record.url?scp=78751506588&partnerID=8YFLogxK
U2 - 10.1039/c004108d
DO - 10.1039/c004108d
M3 - Article
C2 - 20523938
AN - SCOPUS:78751506588
SN - 1742-206X
VL - 6
SP - 1700
EP - 1706
JO - Molecular BioSystems
JF - Molecular BioSystems
IS - 9
ER -