ChIP-chip designs to interrogate the genome of Xenopus embryos for transcription factor binding and epigenetic regulation

Robert C. Akkers, Simon J. Van Heeringen, J. Robert Manak, Roland D. Green, Hendrik G. Stunnenberg, Gert Jan C. Veenstra

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

Background: Chromatin immunoprecipitation combined with genome tile path microarrays or deep sequencing can be used to study genome-wide epigenetic profiles and the transcription factor binding repertoire. Although well studied in a variety of cell lines, these genome-wide profiles have so far been little explored in vertebrate embryos. Principal Findings: Here we report on two genome tile path ChIP-chip designs for interrogating the Xenopus tropicalis genome. In particular, a whole-genome microarray design was used to identify active promoters by close proximity to histone H3 lysine 4 trimethylation. A second microarray design features these experimentally derived promoter regions in addition to currently annotated 5′ ends of genes. These regions truly represent promoters as shown by binding of TBP, a key transcription initiation factor. Conclusions: A whole-genome and a promoter tile path microarray design was developed. Both designs can be used to study epigenetic phenomena and transcription factor binding in developing Xenopus embryos.

Original languageEnglish
Article numbere8820
JournalPLoS ONE
Volume5
Issue number1
DOIs
Publication statusPublished - 21 Jan 2010
Externally publishedYes

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