TY - JOUR
T1 - Clonal acquisition of the Ly49A NK cell receptor is dependent on the trans-acting factor TCF-1
AU - Held, Werner
AU - Kunz, Béatrice
AU - Lowin-Kropf, Bente
AU - Van de Wetering, Marc
AU - Clevers, Hans
N1 - Funding Information:
We thank P. Reichenbach for advice and P. Zaech for expert assistance with flow cytometry, V. Kumar, S. Lemieux, J. Ortaldo, and J. Roland for providing mAbs, and N. Maruyama for the genomic Ly49A clone. We also thank H. R. MacDonald, M. Nabholz, and J.-C. Cerottini for discussion and critical reading of the manuscript. W. H. is the recipient of a START fellowship and supported in part by a grant from the Swiss National Science Foundation.
PY - 1999/10
Y1 - 1999/10
N2 - Families of clonally expressed major histocompatibility complex (MHC) class I-specific receptors provide specificity to and regulate the function of natural killer (NK) cells. One of these receptors, mouse Ly49A, is expressed by 20% of NK cells and inhibits the killing of H-2D(d) but not D(b)-expressing target cells. Here, we show that the trans-acting factor TCF- 1 binds to two sites in the Ly49A promoter and regulates its activity. Moreover, we find that TCF-1 determines the size of the Ly49A NK cell subset in vivo in a dosage-dependent manner. We propose that clonal Ly49A acquisition during NK cell development is regulated by TCF-1.
AB - Families of clonally expressed major histocompatibility complex (MHC) class I-specific receptors provide specificity to and regulate the function of natural killer (NK) cells. One of these receptors, mouse Ly49A, is expressed by 20% of NK cells and inhibits the killing of H-2D(d) but not D(b)-expressing target cells. Here, we show that the trans-acting factor TCF- 1 binds to two sites in the Ly49A promoter and regulates its activity. Moreover, we find that TCF-1 determines the size of the Ly49A NK cell subset in vivo in a dosage-dependent manner. We propose that clonal Ly49A acquisition during NK cell development is regulated by TCF-1.
UR - https://www.scopus.com/pages/publications/0033212750
U2 - 10.1016/S1074-7613(00)80118-1
DO - 10.1016/S1074-7613(00)80118-1
M3 - Article
C2 - 10549625
AN - SCOPUS:0033212750
SN - 1074-7613
VL - 11
SP - 433
EP - 442
JO - Immunity
JF - Immunity
IS - 4
ER -