Cloning and expression of a mouse cDNA encoding p59, an immunophilin that associates with the glucocorticoid receptor

Jacky Schmitt, Jens Pohl, Hendrik G. Stunnenberg

Research output: Contribution to journalArticlepeer-review

28 Citations (Scopus)

Abstract

A cDNA encoding the homologue of the rabbit immunophilin p59 was cloned from a mouse NIH-3T3 cell line library. Antibodies were generated against the N-terminal fragment of the protein produced in bacteria. Western blotting experiments suggest that homologous proteins are present in several other cell lines tested. Production of mouse p59 using recombinant vaccinia viruses resulted in a protein with the expected size of 59 kDa that can interact with the recombinant glucocorticoid receptor, as shown by co-immunoprecipitation experiments.

Original languageEnglish
Pages (from-to)267-271
Number of pages5
JournalGene
Volume132
Issue number2
DOIs
Publication statusPublished - 15 Oct 1993
Externally publishedYes

Keywords

  • FK506-binding protein
  • heat-shock proteins
  • vaccinia virus

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