Abstract
CRISPR-Cas technology has revolutionized biological research and holds great therapeutic potential. Here, we review CRISPR-Cas systems and their latest developments with an emphasis on application to human cells. We also discuss how different CRISPR-based strategies can be used to accomplish a particular genome engineering goal. We then review how different CRISPR tools have been used in genome engineering of human stem cells in vitro, covering both the pluripotent (iPSC/ESC) and somatic adult stem cell fields and, in particular, 3D organoid cultures. Finally, we discuss the progress and challenges associated with CRISPR-based genome editing of human stem cells for therapeutic use.
Original language | English |
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Pages (from-to) | 705-731 |
Number of pages | 27 |
Journal | Cell stem cell |
Volume | 27 |
Issue number | 5 |
DOIs | |
Publication status | Published - 5 Nov 2020 |
Keywords
- CRISPR-Cas Systems/genetics
- Clustered Regularly Interspaced Short Palindromic Repeats/genetics
- Gene Editing
- Genetic Engineering
- Humans
- Organoids