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D-HPLC analysis of the entire FLT3 gene in MLL rearranged and hyperdiploid acute lymphoblastic leukemia

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23 Citations (Scopus)

Abstract

MLL rearranged and hyperdiploid acute lymphoblastic leukemia (ALL) are characterized by high-level FLT3 expression and constitutive FLT3 activation. As known activating FLT3 mutations are often absent in these patients, we screened the entire FLT3 coding sequence in MLL rearranged and hyperdiploid ALL cases for yet unidentified additional genetic alterations using denaturing D-HPLC. Both in MLL rearranged and hyperdiploid ALL we found that a small minority of samples, 7% and 10% respectively, carried genetic alterations. Although some of these alterations may induce FLT3 activation, the majority of these patients carry wild-type FLT3 genes.

Original languageEnglish
Pages (from-to)1565-8
Number of pages4
JournalHaematologica
Volume92
Issue number11
DOIs
Publication statusPublished - Nov 2007
Externally publishedYes

Keywords

  • Chromatography, High Pressure Liquid
  • DNA Mutational Analysis
  • Gene Rearrangement
  • Humans
  • Myeloid-Lymphoid Leukemia Protein/genetics
  • Ploidies
  • Precursor Cell Lymphoblastic Leukemia-Lymphoma/epidemiology
  • fms-Like Tyrosine Kinase 3/genetics

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