Defining minimum genomic regions of imbalance involved in testicular germ cell tumors of adolescents and adults through genome wide microarray analysis of cDNA clones

Alan McIntyre, Brenda Summersgill, Osman Jafer, Sandrine Rodriguez, Gaetano Zafarana, J Wolter Oosterhuis, Ad Jm Gillis, Leendert Looijenga, Colin Cooper, Robert Huddart, Jeremy Clark, Janet Shipley

Research output: Contribution to journalArticlepeer-review

Abstract

Identifying changes in DNA copy number can pinpoint genes that may be involved in tumor development. Here we have defined the smallest overlapping regions of imbalance (SORI) in testicular germ cell tumors other than the 12p region, which has been previously investigated. Definition of the regions was achieved through comparative genomic hybridization (CGH) analysis of a 4559 cDNA clone microarray. A total of 14 SORI were identified, which involved at least five of the 11 samples analysed. Many of these refined regions were previously reported using chromosomal or allelic imbalance studies. The SORI included gain of material from the regions 4q12, 17q21.3, 22q11.23 and Xq22, and loss from 5q33, 11q12.1, 16q22.3 and 22q11. Comparison with parallel chromosomal CGH data supported involvement of most regions. The various SORI span between one and 20 genes and highlight potential oncogenes/tumor suppressor genes to be investigated further.

Original languageEnglish
Pages (from-to)9142-7
Number of pages6
JournalOncogene
Volume23
Issue number56
DOIs
Publication statusPublished - 2 Dec 2004
Externally publishedYes

Keywords

  • Adolescent
  • Adult
  • Chromosome Mapping
  • DNA, Complementary/genetics
  • Germinoma/genetics
  • Humans
  • Male
  • Oligonucleotide Array Sequence Analysis
  • Testicular Neoplasms/genetics

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