Differences between rodent and human cell lines in the amount of integrated DNA after transfection

Jan H.J. Hoeijmakers; Hanny Odijk; Andries Westerveld

doi:10.1016/0014-4827(87)90230-8

Differences between rodent and human cell lines in the amount of integrated DNA after transfection

Jan H.J. Hoeijmakers
, Hanny Odijk
, Andries Westerveld

Research output: Contribution to journal › Article › peer-review

69 Citations (Scopus)

Abstract

The suitability of Chinese hamster and human cell lines for DNA-mediated gene transformation was investigated with respect to two parameters: the average quantity of and the integrity of integrated exogenous DNA fragments. No large differences were observed between most cell lines concerning the extent of fragmentation of the transferred DNA molecules. By contrast, the average number of sequences stably incorporated by the human cells (four lines tested) was 20- to 100-fold lower than the average amount inserted in the five Chinese hamster lines investigated. The very low uptake exhibited by the human cells, ranging from less than 100 up to 500 kb, renders these cells less suitable for transfection with genomic DNA to isolate specific genes.

Original language	English
Pages (from-to)	111-119
Number of pages	9
Journal	Experimental Cell Research
Volume	169
Issue number	1
DOIs	https://doi.org/10.1016/0014-4827(87)90230-8
Publication status	Published - Mar 1987
Externally published	Yes

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title = "Differences between rodent and human cell lines in the amount of integrated DNA after transfection",

abstract = "The suitability of Chinese hamster and human cell lines for DNA-mediated gene transformation was investigated with respect to two parameters: the average quantity of and the integrity of integrated exogenous DNA fragments. No large differences were observed between most cell lines concerning the extent of fragmentation of the transferred DNA molecules. By contrast, the average number of sequences stably incorporated by the human cells (four lines tested) was 20- to 100-fold lower than the average amount inserted in the five Chinese hamster lines investigated. The very low uptake exhibited by the human cells, ranging from less than 100 up to 500 kb, renders these cells less suitable for transfection with genomic DNA to isolate specific genes.",

author = "Hoeijmakers, \{Jan H.J.\} and Hanny Odijk and Andries Westerveld",

note = "Funding Information: This study was supported financially by EURATGM (contract no. BI6-141-NL) and FUNGG, Foundation of Medical Scientific Research in The Netherlands (contract 900-501-091).",

year = "1987",

month = mar,

doi = "10.1016/0014-4827(87)90230-8",

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pages = "111--119",

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T1 - Differences between rodent and human cell lines in the amount of integrated DNA after transfection

AU - Hoeijmakers, Jan H.J.

AU - Odijk, Hanny

AU - Westerveld, Andries

N1 - Funding Information: This study was supported financially by EURATGM (contract no. BI6-141-NL) and FUNGG, Foundation of Medical Scientific Research in The Netherlands (contract 900-501-091).

PY - 1987/3

Y1 - 1987/3

N2 - The suitability of Chinese hamster and human cell lines for DNA-mediated gene transformation was investigated with respect to two parameters: the average quantity of and the integrity of integrated exogenous DNA fragments. No large differences were observed between most cell lines concerning the extent of fragmentation of the transferred DNA molecules. By contrast, the average number of sequences stably incorporated by the human cells (four lines tested) was 20- to 100-fold lower than the average amount inserted in the five Chinese hamster lines investigated. The very low uptake exhibited by the human cells, ranging from less than 100 up to 500 kb, renders these cells less suitable for transfection with genomic DNA to isolate specific genes.

AB - The suitability of Chinese hamster and human cell lines for DNA-mediated gene transformation was investigated with respect to two parameters: the average quantity of and the integrity of integrated exogenous DNA fragments. No large differences were observed between most cell lines concerning the extent of fragmentation of the transferred DNA molecules. By contrast, the average number of sequences stably incorporated by the human cells (four lines tested) was 20- to 100-fold lower than the average amount inserted in the five Chinese hamster lines investigated. The very low uptake exhibited by the human cells, ranging from less than 100 up to 500 kb, renders these cells less suitable for transfection with genomic DNA to isolate specific genes.

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VL - 169

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JO - Experimental Cell Research

JF - Experimental Cell Research

IS - 1

ER -

Differences between rodent and human cell lines in the amount of integrated DNA after transfection

Abstract

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