DNA repair helicase: A component of BTF2 (TFIIH) basic transcription factor

Laurent Schaeffer; Richard Roy; Sandrine Humbert; Vincent Moncollin; Wim Vermeulen; Jan H.J. Hoeijmakers; Pierre Chambon; Jean Marc Egly

doi:10.1126/science.8465201

DNA repair helicase: A component of BTF2 (TFIIH) basic transcription factor

Laurent Schaeffer, Richard Roy, Sandrine Humbert, Vincent Moncollin, Wim Vermeulen, Jan H.J. Hoeijmakers, Pierre Chambon, Jean Marc Egly

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714 Citations (Scopus)

Abstract

The human BTF2 basic transcription factor (also called TFIIH), which is similar to the δ factor in rat and factor b in yeast, is required for class II gene transcription. A strand displacement assay was used to show that highly purified preparation of BTF2 had an adenosine triphosphate-dependent DNA helicase activity, in addition to the previously characterized carboxyl-terminal domain kinase activity. Amino acid sequence analysis of the tryptic digest generated from the 89-kilodalton subunit of BTF2 indicated that this polypeptide corresponded to the ERCC-3 gene product, a presumed helicase implicated in the human DNA excision repair disorders xeroderma pigmentosum and Cockayne's syndrome. These findings suggest that transcription and nucleotide excision repair may share common factors and hence may be considered to be functionally related.

Original language	English
Pages (from-to)	58-63
Number of pages	6
Journal	Science
Volume	260
Issue number	5104
DOIs	https://doi.org/10.1126/science.8465201
Publication status	Published - 1993
Externally published	Yes

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title = "DNA repair helicase: A component of BTF2 (TFIIH) basic transcription factor",

abstract = "The human BTF2 basic transcription factor (also called TFIIH), which is similar to the δ factor in rat and factor b in yeast, is required for class II gene transcription. A strand displacement assay was used to show that highly purified preparation of BTF2 had an adenosine triphosphate-dependent DNA helicase activity, in addition to the previously characterized carboxyl-terminal domain kinase activity. Amino acid sequence analysis of the tryptic digest generated from the 89-kilodalton subunit of BTF2 indicated that this polypeptide corresponded to the ERCC-3 gene product, a presumed helicase implicated in the human DNA excision repair disorders xeroderma pigmentosum and Cockayne's syndrome. These findings suggest that transcription and nucleotide excision repair may share common factors and hence may be considered to be functionally related.",

author = "Laurent Schaeffer and Richard Roy and Sandrine Humbert and Vincent Moncollin and Wim Vermeulen and Hoeijmakers, {Jan H.J.} and Pierre Chambon and Egly, {Jean Marc}",

year = "1993",

doi = "10.1126/science.8465201",

language = "English",

volume = "260",

pages = "58--63",

journal = "Science",

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TY - JOUR

T1 - DNA repair helicase

T2 - A component of BTF2 (TFIIH) basic transcription factor

AU - Schaeffer, Laurent

AU - Roy, Richard

AU - Humbert, Sandrine

AU - Moncollin, Vincent

AU - Vermeulen, Wim

AU - Hoeijmakers, Jan H.J.

AU - Chambon, Pierre

AU - Egly, Jean Marc

PY - 1993

Y1 - 1993

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AB - The human BTF2 basic transcription factor (also called TFIIH), which is similar to the δ factor in rat and factor b in yeast, is required for class II gene transcription. A strand displacement assay was used to show that highly purified preparation of BTF2 had an adenosine triphosphate-dependent DNA helicase activity, in addition to the previously characterized carboxyl-terminal domain kinase activity. Amino acid sequence analysis of the tryptic digest generated from the 89-kilodalton subunit of BTF2 indicated that this polypeptide corresponded to the ERCC-3 gene product, a presumed helicase implicated in the human DNA excision repair disorders xeroderma pigmentosum and Cockayne's syndrome. These findings suggest that transcription and nucleotide excision repair may share common factors and hence may be considered to be functionally related.

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DO - 10.1126/science.8465201

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VL - 260

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JO - Science

JF - Science

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DNA repair helicase: A component of BTF2 (TFIIH) basic transcription factor

Abstract

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