Elucidating miRNA Function in Cancer Biology via the Molecular Genetics' Toolbox

Adam Azlan; Yaashini Rajasegaran; Khor Kang Zi; Aliaa Arina Rosli; Mot Yee Yik; Narazah Mohd Yusoff; Olaf Heidenreich; Emmanuel Jairaj Moses

doi:10.3390/biomedicines10040915

Elucidating miRNA Function in Cancer Biology via the Molecular Genetics' Toolbox

Adam Azlan
, Yaashini Rajasegaran
, Khor Kang Zi
, Aliaa Arina Rosli
, Mot Yee Yik
, Narazah Mohd Yusoff
, Olaf Heidenreich
, Emmanuel Jairaj Moses

Research output: Contribution to journal › Review article › peer-review

6 Citations (Scopus)

Abstract

Micro-RNA (miRNAs) are short non-coding RNAs of about 18-20 nucleotides in length and are implicated in many cellular processes including proliferation, development, differentiation, apoptosis and cell signaling. Furthermore, it is well known that miRNA expression is frequently dysregulated in many cancers. Therefore, this review will highlight the various mechanisms by which microRNAs are dysregulated in cancer. Further highlights include the abundance of molecular genetics tools that are currently available to study miRNA function as well as their advantages and disadvantages with a special focus on various CRISPR/Cas systems This review provides general workflows and some practical considerations when studying miRNA function thus enabling researchers to make informed decisions in regards to the appropriate molecular genetics tool to be utilized for their experiments.

Original language	English
Article number	915
Journal	Biomedicines
Volume	10
Issue number	4
DOIs	https://doi.org/10.3390/biomedicines10040915
Publication status	Published - 15 Apr 2022
Externally published	Yes

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10.3390/biomedicines10040915

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title = "Elucidating miRNA Function in Cancer Biology via the Molecular Genetics' Toolbox",

abstract = "Micro-RNA (miRNAs) are short non-coding RNAs of about 18-20 nucleotides in length and are implicated in many cellular processes including proliferation, development, differentiation, apoptosis and cell signaling. Furthermore, it is well known that miRNA expression is frequently dysregulated in many cancers. Therefore, this review will highlight the various mechanisms by which microRNAs are dysregulated in cancer. Further highlights include the abundance of molecular genetics tools that are currently available to study miRNA function as well as their advantages and disadvantages with a special focus on various CRISPR/Cas systems This review provides general workflows and some practical considerations when studying miRNA function thus enabling researchers to make informed decisions in regards to the appropriate molecular genetics tool to be utilized for their experiments.",

keywords = "CRISPR/Cas9, antagomir, dCas9 modifications, epigenetics, miRNA mimic, miRNASponges, microRNA",

author = "Adam Azlan and Yaashini Rajasegaran and \{Kang Zi\}, Khor and Rosli, \{Aliaa Arina\} and Yik, \{Mot Yee\} and Yusoff, \{Narazah Mohd\} and Olaf Heidenreich and Moses, \{Emmanuel Jairaj\}",

note = "Publisher Copyright: {\textcopyright} 2022 by the authors. Licensee MDPI, Basel, Switzerland.",

year = "2022",

month = apr,

day = "15",

doi = "10.3390/biomedicines10040915",

language = "English",

volume = "10",

journal = "Biomedicines",

issn = "2227-9059",

publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",

number = "4",

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TY - JOUR

T1 - Elucidating miRNA Function in Cancer Biology via the Molecular Genetics' Toolbox

AU - Azlan, Adam

AU - Rajasegaran, Yaashini

AU - Kang Zi, Khor

AU - Rosli, Aliaa Arina

AU - Yik, Mot Yee

AU - Yusoff, Narazah Mohd

AU - Heidenreich, Olaf

AU - Moses, Emmanuel Jairaj

PY - 2022/4/15

Y1 - 2022/4/15

N2 - Micro-RNA (miRNAs) are short non-coding RNAs of about 18-20 nucleotides in length and are implicated in many cellular processes including proliferation, development, differentiation, apoptosis and cell signaling. Furthermore, it is well known that miRNA expression is frequently dysregulated in many cancers. Therefore, this review will highlight the various mechanisms by which microRNAs are dysregulated in cancer. Further highlights include the abundance of molecular genetics tools that are currently available to study miRNA function as well as their advantages and disadvantages with a special focus on various CRISPR/Cas systems This review provides general workflows and some practical considerations when studying miRNA function thus enabling researchers to make informed decisions in regards to the appropriate molecular genetics tool to be utilized for their experiments.

AB - Micro-RNA (miRNAs) are short non-coding RNAs of about 18-20 nucleotides in length and are implicated in many cellular processes including proliferation, development, differentiation, apoptosis and cell signaling. Furthermore, it is well known that miRNA expression is frequently dysregulated in many cancers. Therefore, this review will highlight the various mechanisms by which microRNAs are dysregulated in cancer. Further highlights include the abundance of molecular genetics tools that are currently available to study miRNA function as well as their advantages and disadvantages with a special focus on various CRISPR/Cas systems This review provides general workflows and some practical considerations when studying miRNA function thus enabling researchers to make informed decisions in regards to the appropriate molecular genetics tool to be utilized for their experiments.

KW - CRISPR/Cas9

KW - antagomir

KW - dCas9 modifications

KW - epigenetics

KW - miRNA mimic

KW - miRNASponges

KW - microRNA

UR - https://www.mendeley.com/catalogue/dc4648db-57d9-3b2a-aceb-6fbd487b4892/

U2 - 10.3390/biomedicines10040915

DO - 10.3390/biomedicines10040915

M3 - Review article

C2 - 35453665

SN - 2227-9059

VL - 10

JO - Biomedicines

JF - Biomedicines

IS - 4

M1 - 915

ER -

Elucidating miRNA Function in Cancer Biology via the Molecular Genetics' Toolbox

Abstract

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