ENU mutagenesis to generate genetically modified rat models.

Ruben van Boxtel; Michael N. Gould; Edwin Cuppen; Bart M.G. Smits

doi:10.1007/978-1-60327-389-3_11

ENU mutagenesis to generate genetically modified rat models.

Ruben van Boxtel
, Michael N. Gould
, Edwin Cuppen
, Bart M.G. Smits

Research output: Contribution to journal › Article › peer-review

26 Citations (Scopus)

Abstract

The rat is one of the most preferred model organisms in biomedical research and has been extremely useful for linking physiology and pathology to the genome. However, approaches to genetically modify specific genes in the rat germ line remain relatively scarce. To date, the most efficient approach for generating genetically modified rats has been the target-selected N-ethyl-N-nitrosourea (ENU) mutagenesis-based technology. Here, we describe the detailed protocols for ENU mutagenesis and mutant retrieval in the rat model organism.

Original language	English
Pages (from-to)	151-167
Number of pages	17
Journal	Methods in molecular biology (Clifton, N.J.)
Volume	597
DOIs	https://doi.org/10.1007/978-1-60327-389-3_11
Publication status	Published - 2010
Externally published	Yes

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abstract = "The rat is one of the most preferred model organisms in biomedical research and has been extremely useful for linking physiology and pathology to the genome. However, approaches to genetically modify specific genes in the rat germ line remain relatively scarce. To date, the most efficient approach for generating genetically modified rats has been the target-selected N-ethyl-N-nitrosourea (ENU) mutagenesis-based technology. Here, we describe the detailed protocols for ENU mutagenesis and mutant retrieval in the rat model organism.",

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JF - Methods in molecular biology (Clifton, N.J.)

ER -

ENU mutagenesis to generate genetically modified rat models.

Abstract

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