Evidence for a dynamic role of the linker histone variant H1x during retinoic acid-induced differentiation of NT2 cells

Maryam Shahhoseini; Raha Favaedi; Hossein Baharvand; Vikram Sharma; Hendrik G. Stunnenberg

doi:10.1016/j.febslet.2010.10.041

Evidence for a dynamic role of the linker histone variant H1x during retinoic acid-induced differentiation of NT2 cells

Maryam Shahhoseini, Raha Favaedi, Hossein Baharvand, Vikram Sharma, Hendrik G. Stunnenberg

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

Abstract

The dynamics of chromatin structure are tightly regulated by multiple epigenetic mechanisms such as histone modifications and incorporation of histone variants. In the current work, differentiation of an embryonal carcinoma cell line, NT2, was induced by retinoic acid, and total histone proteins were compared throughout this process. The results showed a significant change in expression level of a variant of H1 histone named H1x. Chromatin immunoprecipitation coupled with real-time PCR analysis demonstrated a preferential incorporation of this protein in the regulatory region of Nanog, a marker gene of stemness that is significantly suppressed in differentiated cells. This finding reveals a dynamic role of H1x in differentiation, and implies a repressive role for this histone variant.

Original language	English
Pages (from-to)	4661-4664
Number of pages	4
Journal	FEBS Letters
Volume	584
Issue number	22
DOIs	https://doi.org/10.1016/j.febslet.2010.10.041
Publication status	Published - 19 Nov 2010
Externally published	Yes

Keywords

Differentiation
H1x
Histone variant
NT2

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10.1016/j.febslet.2010.10.041

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title = "Evidence for a dynamic role of the linker histone variant H1x during retinoic acid-induced differentiation of NT2 cells",

abstract = "The dynamics of chromatin structure are tightly regulated by multiple epigenetic mechanisms such as histone modifications and incorporation of histone variants. In the current work, differentiation of an embryonal carcinoma cell line, NT2, was induced by retinoic acid, and total histone proteins were compared throughout this process. The results showed a significant change in expression level of a variant of H1 histone named H1x. Chromatin immunoprecipitation coupled with real-time PCR analysis demonstrated a preferential incorporation of this protein in the regulatory region of Nanog, a marker gene of stemness that is significantly suppressed in differentiated cells. This finding reveals a dynamic role of H1x in differentiation, and implies a repressive role for this histone variant.",

keywords = "Differentiation, H1x, Histone variant, NT2",

author = "Maryam Shahhoseini and Raha Favaedi and Hossein Baharvand and Vikram Sharma and Stunnenberg, {Hendrik G.}",

note = "Funding Information: We wish to thank Dr. Ghasem Hosseini Salekdeh for his worthwhile guidance and providing protein science facilities. The authors would like to dedicate this paper to the memory of Dr. Saeid Kazemi Ashtiani, the late founder of Royan Institute. This project was financially supported by the grant # 169 of Royan Institute. ",

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doi = "10.1016/j.febslet.2010.10.041",

language = "English",

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T1 - Evidence for a dynamic role of the linker histone variant H1x during retinoic acid-induced differentiation of NT2 cells

AU - Shahhoseini, Maryam

AU - Favaedi, Raha

AU - Baharvand, Hossein

AU - Sharma, Vikram

AU - Stunnenberg, Hendrik G.

N1 - Funding Information: We wish to thank Dr. Ghasem Hosseini Salekdeh for his worthwhile guidance and providing protein science facilities. The authors would like to dedicate this paper to the memory of Dr. Saeid Kazemi Ashtiani, the late founder of Royan Institute. This project was financially supported by the grant # 169 of Royan Institute.

PY - 2010/11/19

Y1 - 2010/11/19

N2 - The dynamics of chromatin structure are tightly regulated by multiple epigenetic mechanisms such as histone modifications and incorporation of histone variants. In the current work, differentiation of an embryonal carcinoma cell line, NT2, was induced by retinoic acid, and total histone proteins were compared throughout this process. The results showed a significant change in expression level of a variant of H1 histone named H1x. Chromatin immunoprecipitation coupled with real-time PCR analysis demonstrated a preferential incorporation of this protein in the regulatory region of Nanog, a marker gene of stemness that is significantly suppressed in differentiated cells. This finding reveals a dynamic role of H1x in differentiation, and implies a repressive role for this histone variant.

AB - The dynamics of chromatin structure are tightly regulated by multiple epigenetic mechanisms such as histone modifications and incorporation of histone variants. In the current work, differentiation of an embryonal carcinoma cell line, NT2, was induced by retinoic acid, and total histone proteins were compared throughout this process. The results showed a significant change in expression level of a variant of H1 histone named H1x. Chromatin immunoprecipitation coupled with real-time PCR analysis demonstrated a preferential incorporation of this protein in the regulatory region of Nanog, a marker gene of stemness that is significantly suppressed in differentiated cells. This finding reveals a dynamic role of H1x in differentiation, and implies a repressive role for this histone variant.

KW - Differentiation

KW - H1x

KW - Histone variant

KW - NT2

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JF - FEBS Letters

IS - 22

ER -

Evidence for a dynamic role of the linker histone variant H1x during retinoic acid-induced differentiation of NT2 cells

Abstract

Keywords

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