Expression of AC133 and CD117 on candidate normal stem cell populations in childhood B-cell precursor acute lymphoblastic leukaemia

G Baersch, M Baumann, J Ritter, H Jürgens, J Vormoor

Research output: Contribution to journalArticlepeer-review

Abstract

To identify residual candidate normal progenitor/stem cell populations in childhood B-cell precursor acute lymphoblastic leukaemia (ALL), expression of AC133 and CD117 was analysed on the leukaemic cell clone and on immature B-lineage-negative CD34+CD19- bone marrow cells. 10/25 patients (40%) had no detectable expression of AC133 within the leukaemic cell clone. 24/26 patients (92%) lacked expression of CD117 on the leukaemic blast cell population. In contrast, a distinct AC133-positive cell population was found in 8/8 children with AC133-negative ALL and a CD117-positive cell population could be identified in 12/12 children with CD117-negative ALL, within the CD34+CD19- progenitor/stem cell compartment. These observations provide further evidence that in B-cell precursor ALL, unlike in acute myelogenous leukaemia, it may be possible to distinguish residual normal progenitor/stem cells from the leukaemic cell clone.

Original languageEnglish
Pages (from-to)572-80
Number of pages9
JournalBritish journal of haematology
Volume107
Issue number3
DOIs
Publication statusPublished - Dec 1999
Externally publishedYes

Keywords

  • AC133 Antigen
  • Antigens, CD
  • Antigens, CD19/metabolism
  • Antigens, CD34/metabolism
  • Child
  • Flow Cytometry
  • Glycoproteins/metabolism
  • Humans
  • Leukocyte Common Antigens/metabolism
  • Peptides/metabolism
  • Precursor B-Cell Lymphoblastic Leukemia-Lymphoma/metabolism
  • Precursor Cell Lymphoblastic Leukemia-Lymphoma/metabolism
  • Proto-Oncogene Proteins c-kit/metabolism
  • Reverse Transcriptase Polymerase Chain Reaction/methods
  • Stem Cells/metabolism

Fingerprint

Dive into the research topics of 'Expression of AC133 and CD117 on candidate normal stem cell populations in childhood B-cell precursor acute lymphoblastic leukaemia'. Together they form a unique fingerprint.

Cite this