Expression of CD44 and CD35 during normal and myelodysplastic erythropoiesis

Paula Laranjeira; Raquel Rodrigues; Tiago Carvalheiro; Conceição Constanço; Helena Vitória; Sergio Matarraz; Hélder Trindade; Alberto Órfão; Artur Paiva

doi:10.1016/j.leukres.2014.12.009

Expression of CD44 and CD35 during normal and myelodysplastic erythropoiesis

Paula Laranjeira, Raquel Rodrigues, Tiago Carvalheiro, Conceição Constanço, Helena Vitória, Sergio Matarraz, Hélder Trindade, Alberto Órfão, Artur Paiva

Research output: Contribution to journal › Article › peer-review

21 Citations (Scopus)

Abstract

Erythroid dysplasia is a common feature of myelodysplastic syndromes (MDS). Currently available information about the immunophenotypic features of normal and dysplastic erythropoiesis is scarce and restricted to relatively few markers. Here we studied the expression of CD117, CD35 and CD44 throughout the normal (n=16) and dysplastic (n=48) bone marrow erythroid maturation. CD35 emerged as an early marker of CD34(+) erythroid-committed precursors, which is expressed before CD105 and remains positive thereafter. MDS patients (with and without morphologic dyserythropoiesis) displayed overall increased expression of CD44, associated with slight alterations on CD35 expression, suggesting that phenotypic alterations in MDS may precede morphologic dysplasia. In turn, MDS patients with anemia showed increased expression of CD117.

Original language	English
Pages (from-to)	361-70
Number of pages	10
Journal	Leukemia research
Volume	39
Issue number	3
DOIs	https://doi.org/10.1016/j.leukres.2014.12.009
Publication status	Published - Mar 2015
Externally published	Yes

Keywords

Aged
Biomarkers, Tumor/metabolism
Bone Marrow/metabolism
Case-Control Studies
Erythroid Precursor Cells/metabolism
Erythropoiesis
Female
Flow Cytometry
Follow-Up Studies
Humans
Hyaluronan Receptors/metabolism
Immunophenotyping
Male
Middle Aged
Myelodysplastic Syndromes/metabolism
Neoplasm Staging
Phenotype
Prognosis
Receptors, Complement 3b/metabolism

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10.1016/j.leukres.2014.12.009

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title = "Expression of CD44 and CD35 during normal and myelodysplastic erythropoiesis",

abstract = "Erythroid dysplasia is a common feature of myelodysplastic syndromes (MDS). Currently available information about the immunophenotypic features of normal and dysplastic erythropoiesis is scarce and restricted to relatively few markers. Here we studied the expression of CD117, CD35 and CD44 throughout the normal (n=16) and dysplastic (n=48) bone marrow erythroid maturation. CD35 emerged as an early marker of CD34(+) erythroid-committed precursors, which is expressed before CD105 and remains positive thereafter. MDS patients (with and without morphologic dyserythropoiesis) displayed overall increased expression of CD44, associated with slight alterations on CD35 expression, suggesting that phenotypic alterations in MDS may precede morphologic dysplasia. In turn, MDS patients with anemia showed increased expression of CD117. ",

keywords = "Aged, Biomarkers, Tumor/metabolism, Bone Marrow/metabolism, Case-Control Studies, Erythroid Precursor Cells/metabolism, Erythropoiesis, Female, Flow Cytometry, Follow-Up Studies, Humans, Hyaluronan Receptors/metabolism, Immunophenotyping, Male, Middle Aged, Myelodysplastic Syndromes/metabolism, Neoplasm Staging, Phenotype, Prognosis, Receptors, Complement 3b/metabolism",

author = "Paula Laranjeira and Raquel Rodrigues and Tiago Carvalheiro and Concei{\c c}{\~a}o Constan{\c c}o and Helena Vit{\'o}ria and Sergio Matarraz and H{\'e}lder Trindade and Alberto {\'O}rf{\~a}o and Artur Paiva",

year = "2015",

month = mar,

doi = "10.1016/j.leukres.2014.12.009",

language = "English",

volume = "39",

pages = "361--70",

journal = "Leukemia research",

issn = "0145-2126",

publisher = "Elsevier Ltd.",

number = "3",

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TY - JOUR

T1 - Expression of CD44 and CD35 during normal and myelodysplastic erythropoiesis

AU - Laranjeira, Paula

AU - Rodrigues, Raquel

AU - Carvalheiro, Tiago

AU - Constanço, Conceição

AU - Vitória, Helena

AU - Matarraz, Sergio

AU - Trindade, Hélder

AU - Órfão, Alberto

AU - Paiva, Artur

PY - 2015/3

Y1 - 2015/3

N2 - Erythroid dysplasia is a common feature of myelodysplastic syndromes (MDS). Currently available information about the immunophenotypic features of normal and dysplastic erythropoiesis is scarce and restricted to relatively few markers. Here we studied the expression of CD117, CD35 and CD44 throughout the normal (n=16) and dysplastic (n=48) bone marrow erythroid maturation. CD35 emerged as an early marker of CD34(+) erythroid-committed precursors, which is expressed before CD105 and remains positive thereafter. MDS patients (with and without morphologic dyserythropoiesis) displayed overall increased expression of CD44, associated with slight alterations on CD35 expression, suggesting that phenotypic alterations in MDS may precede morphologic dysplasia. In turn, MDS patients with anemia showed increased expression of CD117.

AB - Erythroid dysplasia is a common feature of myelodysplastic syndromes (MDS). Currently available information about the immunophenotypic features of normal and dysplastic erythropoiesis is scarce and restricted to relatively few markers. Here we studied the expression of CD117, CD35 and CD44 throughout the normal (n=16) and dysplastic (n=48) bone marrow erythroid maturation. CD35 emerged as an early marker of CD34(+) erythroid-committed precursors, which is expressed before CD105 and remains positive thereafter. MDS patients (with and without morphologic dyserythropoiesis) displayed overall increased expression of CD44, associated with slight alterations on CD35 expression, suggesting that phenotypic alterations in MDS may precede morphologic dysplasia. In turn, MDS patients with anemia showed increased expression of CD117.

KW - Aged

KW - Biomarkers, Tumor/metabolism

KW - Bone Marrow/metabolism

KW - Case-Control Studies

KW - Erythroid Precursor Cells/metabolism

KW - Erythropoiesis

KW - Female

KW - Flow Cytometry

KW - Follow-Up Studies

KW - Humans

KW - Hyaluronan Receptors/metabolism

KW - Immunophenotyping

KW - Male

KW - Middle Aged

KW - Myelodysplastic Syndromes/metabolism

KW - Neoplasm Staging

KW - Phenotype

KW - Prognosis

KW - Receptors, Complement 3b/metabolism

U2 - 10.1016/j.leukres.2014.12.009

DO - 10.1016/j.leukres.2014.12.009

M3 - Article

C2 - 25582385

SN - 0145-2126

VL - 39

SP - 361

EP - 370

JO - Leukemia research

JF - Leukemia research

IS - 3

ER -

Expression of CD44 and CD35 during normal and myelodysplastic erythropoiesis

Abstract

Keywords

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