TY - JOUR
T1 - Expression of GAD67 and novel GAD67 splice variants during human fetal pancreas development
T2 - GAD67 expression in the fetal pancreas
AU - Korpershoek, Esther
AU - Verwest, Aart M.
AU - Ijzendoorn, Ynske
AU - Rottier, Robbert
AU - Drexhage, Hemmo A.
AU - De Krijger, Ronald R.
N1 - Funding Information:
Acknowledgments We are grateful to the Erasmus MC Tissue Bank (Department of Pathology, Rotterdam, The Netherlands) for providing human fetal and adult frozen pancreas tissue. We gratefully acknowledge Frank van der Panne (Department of Pathology, Josephine Nefkens Institute, Erasmus MC, Rotterdam, The Netherlands) for his assistance with the generation of the figures. This study was supported by a grant from the European Union QLRT-1999-00276 (MONODIAB). The authors declare that there is no conflict of interest that would prejudice the impartiality of this scientific work.
PY - 2007/3
Y1 - 2007/3
N2 - Glutamic acid decarboxylase (GAD) is a major inhibitory neurotransmitter in the brain, which catalyses the reaction of L: -glutamate to γ-aminobutyric acid. There are two isoforms of GAD, a 65-kDa form and a 67-kDa form, which are encoded by two different genes. As previous studies suggested a role for GAD67 splice variants during fetal pancreas development, we have investigated the mRNA expression of GAD67 and GAD67 splice variants in a series of 14 human fetal pancreases between 14 weeks gestation and term and in adult control pancreases by RT-PCR. In this study, we demonstrate mRNA expression of GAD67 and four GAD67 splice variants, including GAD25, in human fetal and adult specimens. Some of the splice variants, including various proportions of exon 7 or a new exon between exons 6 and 7, have not been described before in the human pancreas. We speculate that the expression of these GAD67 splice variants might be related to human fetal pancreas development.
AB - Glutamic acid decarboxylase (GAD) is a major inhibitory neurotransmitter in the brain, which catalyses the reaction of L: -glutamate to γ-aminobutyric acid. There are two isoforms of GAD, a 65-kDa form and a 67-kDa form, which are encoded by two different genes. As previous studies suggested a role for GAD67 splice variants during fetal pancreas development, we have investigated the mRNA expression of GAD67 and GAD67 splice variants in a series of 14 human fetal pancreases between 14 weeks gestation and term and in adult control pancreases by RT-PCR. In this study, we demonstrate mRNA expression of GAD67 and four GAD67 splice variants, including GAD25, in human fetal and adult specimens. Some of the splice variants, including various proportions of exon 7 or a new exon between exons 6 and 7, have not been described before in the human pancreas. We speculate that the expression of these GAD67 splice variants might be related to human fetal pancreas development.
KW - Fetal development
KW - GAD
KW - Human development
KW - Pancreas alternative splicing
UR - http://www.scopus.com/inward/record.url?scp=34548359232&partnerID=8YFLogxK
U2 - 10.1007/s12022-007-0003-y
DO - 10.1007/s12022-007-0003-y
M3 - Article
C2 - 17652798
AN - SCOPUS:34548359232
SN - 1046-3976
VL - 18
SP - 31
EP - 36
JO - Endocrine Pathology
JF - Endocrine Pathology
IS - 1
ER -