TY - JOUR
T1 - Expression profiling after prolonged experimental febrile seizures in mice suggests structural remodeling in the hippocampus
AU - Jongbloets, Bart C.
AU - Van Gassen, Koen L.I.
AU - Kan, Anne A.
AU - Olde Engberink, Anneke H.O.
AU - De Wit, Marina
AU - Wolterink-Donselaar, Inge G.
AU - Groot Koerkamp, Marian J.A.
AU - Van Nieuwenhuizen, Onno
AU - Holstege, Frank C.P.
AU - De Graan, Pierre N.E.
N1 - Publisher Copyright:
© 2015 Jongbloets et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
PY - 2015/12/1
Y1 - 2015/12/1
N2 - Febrile seizures are the most prevalent type of seizures among children up to 5 years of age (2-4% ofWestern-European children). Complex febrile seizures are associated with an increased risk to develop temporal lobe epilepsy. To investigate short-and long-Term effects of experimental febrile seizures (eFS), we induced eFS in highly febrile convulsionsusceptible C57BL/6J mice at post-natal day 10 by exposure to hyperthermia (HT) and compared them to normotherm-exposed (NT) mice.We detected structural re-organization in the hippocampus 14 days after eFS. To identify molecular candidates, which entrain this structural re-organization, we investigated temporal changes in mRNA expression profiles eFS 1 hour to 56 days after eFS.We identified 931 regulated genes and profiled several candidates using in situ hybridization and histology at 3 and 14 days after eFS. This is the first study to report genome-wide transcriptome analysis after eFS in mice.We identify temporal regulation of multiple processes, such as stress-, immune-and inflammatory responses, glia activation, glutamate-glutamine cycle and myelination. Identification of the short-and long-Term changes after eFS is important to elucidate the mechanisms contributing to epileptogenesis.
AB - Febrile seizures are the most prevalent type of seizures among children up to 5 years of age (2-4% ofWestern-European children). Complex febrile seizures are associated with an increased risk to develop temporal lobe epilepsy. To investigate short-and long-Term effects of experimental febrile seizures (eFS), we induced eFS in highly febrile convulsionsusceptible C57BL/6J mice at post-natal day 10 by exposure to hyperthermia (HT) and compared them to normotherm-exposed (NT) mice.We detected structural re-organization in the hippocampus 14 days after eFS. To identify molecular candidates, which entrain this structural re-organization, we investigated temporal changes in mRNA expression profiles eFS 1 hour to 56 days after eFS.We identified 931 regulated genes and profiled several candidates using in situ hybridization and histology at 3 and 14 days after eFS. This is the first study to report genome-wide transcriptome analysis after eFS in mice.We identify temporal regulation of multiple processes, such as stress-, immune-and inflammatory responses, glia activation, glutamate-glutamine cycle and myelination. Identification of the short-and long-Term changes after eFS is important to elucidate the mechanisms contributing to epileptogenesis.
UR - https://www.scopus.com/pages/publications/84956951681
U2 - 10.1371/journal.pone.0145247
DO - 10.1371/journal.pone.0145247
M3 - Article
C2 - 26684451
AN - SCOPUS:84956951681
SN - 1932-6203
VL - 10
JO - PloS one
JF - PloS one
IS - 12
M1 - e0145247
ER -