Fibronectin is a hypoxia-independent target of the tumor suppressor VHL

Hans A.R. Bluyssen; Martijn P.J.K. Lolkema; Moniek Van Beest; Michelle Boone; Cristel M.J.T. Snijckers; Maartje Los; Martijn F.B.G. Gebbink; Branko Braam; Frank C.P. Holstege; Rachel H. Giles; Emile E. Voest

doi:10.1016/S0014-5793(03)01392-9

Fibronectin is a hypoxia-independent target of the tumor suppressor VHL

Hans A.R. Bluyssen, Martijn P.J.K. Lolkema, Moniek Van Beest, Michelle Boone, Cristel M.J.T. Snijckers, Maartje Los, Martijn F.B.G. Gebbink, Branko Braam, Frank C.P. Holstege, Rachel H. Giles, Emile E. Voest

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44 Citations (Scopus)

Abstract

The von Hippel-Lindau (VHL) tumor suppressor gene regulates the extracellular matrix by controlling fibronectin deposition. To identify novel VHL target genes, we subjected mRNA from VHL-deficient RCC cells (786-0-pRC) and a transfectant re-expressing wildtype VHL (786-0-VHL) to differential expression profiling. Among the differentially expressed genes, we detected that fibronectin is upregulated in the presence of VHL, while it is not affected by hypoxia. Thus regulation of fibronectin deposition by VHL occurs at the transcriptional level, irrespective of oxygen levels.

Original language	English
Pages (from-to)	137-142
Number of pages	6
Journal	FEBS Letters
Volume	556
Issue number	1-3
DOIs	https://doi.org/10.1016/S0014-5793(03)01392-9
Publication status	Published - 2 Jan 2004
Externally published	Yes

Keywords

Fibronectin
Hypoxia
Microarray
Renal cell carcinoma
VHL

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10.1016/S0014-5793(03)01392-9

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@article{b5d242a4fe9a4477bcdfd8f4751010e4,

title = "Fibronectin is a hypoxia-independent target of the tumor suppressor VHL",

abstract = "The von Hippel-Lindau (VHL) tumor suppressor gene regulates the extracellular matrix by controlling fibronectin deposition. To identify novel VHL target genes, we subjected mRNA from VHL-deficient RCC cells (786-0-pRC) and a transfectant re-expressing wildtype VHL (786-0-VHL) to differential expression profiling. Among the differentially expressed genes, we detected that fibronectin is upregulated in the presence of VHL, while it is not affected by hypoxia. Thus regulation of fibronectin deposition by VHL occurs at the transcriptional level, irrespective of oxygen levels.",

keywords = "Fibronectin, Hypoxia, Microarray, Renal cell carcinoma, VHL",

author = "Bluyssen, {Hans A.R.} and Lolkema, {Martijn P.J.K.} and {Van Beest}, Moniek and Michelle Boone and Snijckers, {Cristel M.J.T.} and Maartje Los and Gebbink, {Martijn F.B.G.} and Branko Braam and Holstege, {Frank C.P.} and Giles, {Rachel H.} and Voest, {Emile E.}",

note = "Funding Information: We thank Maarten Pennings for performing the fibronectin ELISAs and Germa Tooten for IGFBP3 quantifications. This study was supported in part by the Dutch Cancer Society (Grants UU 1999-1879 and UU 1999-2114 to E.V.) and in part by the Dutch Scientific Counsel (NWO) (Grant 920-03-179 to M.L. and E.V.).",

year = "2004",

month = jan,

day = "2",

doi = "10.1016/S0014-5793(03)01392-9",

language = "English",

volume = "556",

pages = "137--142",

journal = "FEBS Letters",

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publisher = "John Wiley and Sons Inc.",

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T1 - Fibronectin is a hypoxia-independent target of the tumor suppressor VHL

AU - Bluyssen, Hans A.R.

AU - Lolkema, Martijn P.J.K.

AU - Van Beest, Moniek

AU - Boone, Michelle

AU - Snijckers, Cristel M.J.T.

AU - Los, Maartje

AU - Gebbink, Martijn F.B.G.

AU - Braam, Branko

AU - Holstege, Frank C.P.

AU - Giles, Rachel H.

AU - Voest, Emile E.

N1 - Funding Information: We thank Maarten Pennings for performing the fibronectin ELISAs and Germa Tooten for IGFBP3 quantifications. This study was supported in part by the Dutch Cancer Society (Grants UU 1999-1879 and UU 1999-2114 to E.V.) and in part by the Dutch Scientific Counsel (NWO) (Grant 920-03-179 to M.L. and E.V.).

PY - 2004/1/2

Y1 - 2004/1/2

N2 - The von Hippel-Lindau (VHL) tumor suppressor gene regulates the extracellular matrix by controlling fibronectin deposition. To identify novel VHL target genes, we subjected mRNA from VHL-deficient RCC cells (786-0-pRC) and a transfectant re-expressing wildtype VHL (786-0-VHL) to differential expression profiling. Among the differentially expressed genes, we detected that fibronectin is upregulated in the presence of VHL, while it is not affected by hypoxia. Thus regulation of fibronectin deposition by VHL occurs at the transcriptional level, irrespective of oxygen levels.

AB - The von Hippel-Lindau (VHL) tumor suppressor gene regulates the extracellular matrix by controlling fibronectin deposition. To identify novel VHL target genes, we subjected mRNA from VHL-deficient RCC cells (786-0-pRC) and a transfectant re-expressing wildtype VHL (786-0-VHL) to differential expression profiling. Among the differentially expressed genes, we detected that fibronectin is upregulated in the presence of VHL, while it is not affected by hypoxia. Thus regulation of fibronectin deposition by VHL occurs at the transcriptional level, irrespective of oxygen levels.

KW - Fibronectin

KW - Hypoxia

KW - Microarray

KW - Renal cell carcinoma

KW - VHL

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U2 - 10.1016/S0014-5793(03)01392-9

DO - 10.1016/S0014-5793(03)01392-9

M3 - Article

C2 - 14706840

AN - SCOPUS:9144232529

SN - 0014-5793

VL - 556

SP - 137

EP - 142

JO - FEBS Letters

JF - FEBS Letters

IS - 1-3

ER -

Fibronectin is a hypoxia-independent target of the tumor suppressor VHL

Abstract

Keywords

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