FoxM1 is required for execution of the mitotic programme and chromosome stability

Jamila Laoukili; Matthijs R.H. Kooistra; Alexandra Brás; Jos Kauw; Ron M. Kerkhoven; Ashby Morrison; Hans Clevers; René H. Medema

doi:10.1038/ncb1217

FoxM1 is required for execution of the mitotic programme and chromosome stability

Jamila Laoukili, Matthijs R.H. Kooistra, Alexandra Brás, Jos Kauw, Ron M. Kerkhoven, Ashby Morrison, Hans Clevers, René H. Medema

Research output: Contribution to journal › Article › peer-review

693 Citations (Scopus)

Abstract

Transcriptional induction of cell-cycle regulatory proteins ensures proper timing of subsequent cell-cycle events. Here we show that the Forkhead transcription factor FoxM1 regulates expression of many G2-specific genes and is essential for chromosome stability. Loss of FoxM1 leads to pleiotropic cell-cycle defects, including a delay in G2, chromosome mis-segregation and frequent failure of cytokinesis. We show that transcriptional activation of cyclin B by FoxM1 is essential for timely mitotic entry, whereas CENP-F, another direct target of FoxM1 identified here, is essential for precise functioning of the mitotic spindle checkpoint. Thus, our data uncover a transcriptional cluster regulated by FoxM1 that is essential for proper mitotic progression.

Original language	English
Pages (from-to)	126-136
Number of pages	11
Journal	Nature Cell Biology
Volume	7
Issue number	2
DOIs	https://doi.org/10.1038/ncb1217
Publication status	Published - Feb 2005
Externally published	Yes

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10.1038/ncb1217

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@article{d57444026902495f858491fa23c9377b,

title = "FoxM1 is required for execution of the mitotic programme and chromosome stability",

abstract = "Transcriptional induction of cell-cycle regulatory proteins ensures proper timing of subsequent cell-cycle events. Here we show that the Forkhead transcription factor FoxM1 regulates expression of many G2-specific genes and is essential for chromosome stability. Loss of FoxM1 leads to pleiotropic cell-cycle defects, including a delay in G2, chromosome mis-segregation and frequent failure of cytokinesis. We show that transcriptional activation of cyclin B by FoxM1 is essential for timely mitotic entry, whereas CENP-F, another direct target of FoxM1 identified here, is essential for precise functioning of the mitotic spindle checkpoint. Thus, our data uncover a transcriptional cluster regulated by FoxM1 that is essential for proper mitotic progression.",

author = "Jamila Laoukili and Kooistra, {Matthijs R.H.} and Alexandra Br{\'a}s and Jos Kauw and Kerkhoven, {Ron M.} and Ashby Morrison and Hans Clevers and Medema, {Ren{\'e} H.}",

note = "Funding Information: We would like to thank the other members of the laboratory for helpful discussions and daily support. We would also like to thank M. Heimerikx and A. Velds for expert technical assistance with microarray analyses, R. Beijersbergen for the retro-pSuper-CENP-F, S. Taylor for the anti-BubR1 antibody and helpful discussion, R. Herrera for help with ChIP assays, and L. Oomen and L. Brocks for support with confocal microscopy. The authors acknowledge support from the Dutch Cancer Society and from the FCT (to A.B).",

year = "2005",

month = feb,

doi = "10.1038/ncb1217",

language = "English",

volume = "7",

pages = "126--136",

journal = "Nature Cell Biology",

issn = "1465-7392",

publisher = "Nature Research",

number = "2",

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T1 - FoxM1 is required for execution of the mitotic programme and chromosome stability

AU - Laoukili, Jamila

AU - Kooistra, Matthijs R.H.

AU - Brás, Alexandra

AU - Kauw, Jos

AU - Kerkhoven, Ron M.

AU - Morrison, Ashby

AU - Clevers, Hans

AU - Medema, René H.

N1 - Funding Information: We would like to thank the other members of the laboratory for helpful discussions and daily support. We would also like to thank M. Heimerikx and A. Velds for expert technical assistance with microarray analyses, R. Beijersbergen for the retro-pSuper-CENP-F, S. Taylor for the anti-BubR1 antibody and helpful discussion, R. Herrera for help with ChIP assays, and L. Oomen and L. Brocks for support with confocal microscopy. The authors acknowledge support from the Dutch Cancer Society and from the FCT (to A.B).

PY - 2005/2

Y1 - 2005/2

N2 - Transcriptional induction of cell-cycle regulatory proteins ensures proper timing of subsequent cell-cycle events. Here we show that the Forkhead transcription factor FoxM1 regulates expression of many G2-specific genes and is essential for chromosome stability. Loss of FoxM1 leads to pleiotropic cell-cycle defects, including a delay in G2, chromosome mis-segregation and frequent failure of cytokinesis. We show that transcriptional activation of cyclin B by FoxM1 is essential for timely mitotic entry, whereas CENP-F, another direct target of FoxM1 identified here, is essential for precise functioning of the mitotic spindle checkpoint. Thus, our data uncover a transcriptional cluster regulated by FoxM1 that is essential for proper mitotic progression.

AB - Transcriptional induction of cell-cycle regulatory proteins ensures proper timing of subsequent cell-cycle events. Here we show that the Forkhead transcription factor FoxM1 regulates expression of many G2-specific genes and is essential for chromosome stability. Loss of FoxM1 leads to pleiotropic cell-cycle defects, including a delay in G2, chromosome mis-segregation and frequent failure of cytokinesis. We show that transcriptional activation of cyclin B by FoxM1 is essential for timely mitotic entry, whereas CENP-F, another direct target of FoxM1 identified here, is essential for precise functioning of the mitotic spindle checkpoint. Thus, our data uncover a transcriptional cluster regulated by FoxM1 that is essential for proper mitotic progression.

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JO - Nature Cell Biology

JF - Nature Cell Biology

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ER -

FoxM1 is required for execution of the mitotic programme and chromosome stability

Abstract

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