Frequency and functional activity of Th17, Tc17 and other T-cell subsets in Systemic Lupus Erythematosus

Ana Henriques, Luís Inês, Maura Couto, Susana Pedreiro, Catarina Santos, Mariana Magalhães, Paulo Santos, Isabel Velada, Anabela Almeida, Tiago Carvalheiro, Paula Laranjeira, José Mário Morgado, Maria Luísa Pais, José António Pereira da Silva, Artur Paiva

Research output: Contribution to journalArticlepeer-review

Abstract

To compare frequency and functional activity of peripheral blood (PB) Th(c)17, Th(c)1 and Treg cells and the amount of type 2 cytokines mRNA we recruited SLE patients in active (n=15) and inactive disease (n=19) and healthy age- and gender-matched controls (n=15). The study of Th(c)17, Th(c)1 and Treg cells was done by flow cytometry and cytokine mRNA by real-time PCR. Compared to NC, SLE patients present an increased proportion of Th(c)17 cells, but with lower amounts of IL-17 per cell and also a decreased frequency of Treg, but with increased production of TGF-beta and FoxP3 mRNA. Iotan active compared to inactive SLE, there is a marked decreased in frequency of Th(c)1 cells, an increased production of type 2 cytokines mRNA and a distinct functional profile of Th(c)17 cells. Our findings suggest a functional disequilibrium of T-cell subsets in SLE which may contribute to the inflammatory process and disease pathogenesis.

Original languageEnglish
Pages (from-to)97-103
Number of pages7
JournalCellular immunology
Volume264
Issue number1
DOIs
Publication statusPublished - 2010
Externally publishedYes

Keywords

  • Adult
  • Antigens, CD/biosynthesis
  • Cell Count
  • Cell Separation
  • Female
  • Flow Cytometry
  • Forkhead Transcription Factors/biosynthesis
  • Gene Expression Regulation
  • Humans
  • Interleukin-17/biosynthesis
  • Lupus Erythematosus, Systemic/immunology
  • Male
  • Reverse Transcriptase Polymerase Chain Reaction
  • T-Lymphocyte Subsets/immunology
  • T-Lymphocytes, Cytotoxic/immunology
  • T-Lymphocytes, Regulatory/immunology
  • Th2 Cells/immunology
  • Transforming Growth Factor beta/biosynthesis

Fingerprint

Dive into the research topics of 'Frequency and functional activity of Th17, Tc17 and other T-cell subsets in Systemic Lupus Erythematosus'. Together they form a unique fingerprint.

Cite this