Gene silencing by RNAi in mammalian cells

Frida Ponthan, Narazah Mohd Yusoff, Natalia Martinez Soria, Olaf Heidenreich, Kelly Coffey

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)


This unit provides information how to use short interfering RNA (siRNA) for sequence-specific gene silencing in mammalian cells. Several methods for siRNA generation and optimization, as well as recommendations for cell transfection and transduction, are presented.

Original languageEnglish
Pages (from-to)26.2.1-26.2.17
JournalCurrent Protocols in Molecular Biology
Publication statusPublished - 2015
Externally publishedYes


  • Electroporation
  • Lentiviral transduction
  • RNA interference
  • shRNA
  • siRNA
  • Transfection


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