Gene silencing by RNAi in mammalian cells

Frida Ponthan; Narazah Mohd Yusoff; Natalia Martinez Soria; Olaf Heidenreich; Kelly Coffey

doi:10.1002/0471142727.mb2602s111

Gene silencing by RNAi in mammalian cells

Frida Ponthan, Narazah Mohd Yusoff, Natalia Martinez Soria, Olaf Heidenreich, Kelly Coffey

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

This unit provides information how to use short interfering RNA (siRNA) for sequence-specific gene silencing in mammalian cells. Several methods for siRNA generation and optimization, as well as recommendations for cell transfection and transduction, are presented.

Original language	English
Pages (from-to)	26.2.1-26.2.17
Journal	Current Protocols in Molecular Biology
Volume	2015
DOIs	https://doi.org/10.1002/0471142727.mb2602s111
Publication status	Published - 2015
Externally published	Yes

Keywords

Electroporation
Lentiviral transduction
RNA interference
shRNA
siRNA
Transfection

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10.1002/0471142727.mb2602s111

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@article{766b1e68d9dc408bb0d9d2ff943ea9c9,

title = "Gene silencing by RNAi in mammalian cells",

abstract = "This unit provides information how to use short interfering RNA (siRNA) for sequence-specific gene silencing in mammalian cells. Several methods for siRNA generation and optimization, as well as recommendations for cell transfection and transduction, are presented.",

keywords = "Electroporation, Lentiviral transduction, RNA interference, shRNA, siRNA, Transfection",

author = "Frida Ponthan and Yusoff, {Narazah Mohd} and Soria, {Natalia Martinez} and Olaf Heidenreich and Kelly Coffey",

note = "Publisher Copyright: {\textcopyright} 2015 by John Wiley & Sons, Inc.",

year = "2015",

doi = "10.1002/0471142727.mb2602s111",

language = "English",

volume = "2015",

pages = "26.2.1--26.2.17",

journal = "Current Protocols in Molecular Biology",

issn = "1934-3639",

publisher = "John Wiley & Sons Inc.",

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TY - JOUR

T1 - Gene silencing by RNAi in mammalian cells

AU - Ponthan, Frida

AU - Yusoff, Narazah Mohd

AU - Soria, Natalia Martinez

AU - Heidenreich, Olaf

AU - Coffey, Kelly

PY - 2015

Y1 - 2015

N2 - This unit provides information how to use short interfering RNA (siRNA) for sequence-specific gene silencing in mammalian cells. Several methods for siRNA generation and optimization, as well as recommendations for cell transfection and transduction, are presented.

AB - This unit provides information how to use short interfering RNA (siRNA) for sequence-specific gene silencing in mammalian cells. Several methods for siRNA generation and optimization, as well as recommendations for cell transfection and transduction, are presented.

KW - Electroporation

KW - Lentiviral transduction

KW - RNA interference

KW - shRNA

KW - siRNA

KW - Transfection

UR - http://www.scopus.com/inward/record.url?scp=84949222011&partnerID=8YFLogxK

U2 - 10.1002/0471142727.mb2602s111

DO - 10.1002/0471142727.mb2602s111

M3 - Article

C2 - 26131850

AN - SCOPUS:84949222011

SN - 1934-3639

VL - 2015

SP - 26.2.1-26.2.17

JO - Current Protocols in Molecular Biology

JF - Current Protocols in Molecular Biology

ER -

Gene silencing by RNAi in mammalian cells

Abstract

Keywords

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