Genome-wide binding of MBD2 reveals strong preference for highly methylated loci

Roberta Menafra, Arie B. Brinkman, Filomena Matarese, Gianluigi Franci, Stefanie J.J. Bartels, Luan Nguyen, Takashi Shimbo, Paul A. Wade, Nina C. Hubner, Hendrik G. Stunnenberg

Research output: Contribution to journalArticlepeer-review

38 Citations (Scopus)

Abstract

MBD2 is a subunit of the NuRD complex that is postulated to mediate gene repression via recruitment of the complex to methylated DNA. In this study we adopted an MBD2 tagging-approach to study its genome wide binding characteristics. We show that in vivo MBD2 is mainly recruited to CpG island promoters that are highly methylated. Interestingly, MBD2 binds around 1 kb downstream of the transcription start site of a subset of ∼400 CpG island promoters that are characterized by the presence of active histone marks, RNA polymerase II (Pol2) and low to medium gene expression levels and H3K36me3 deposition. These tagged-MBD2 binding sites in MCF-7 show increased methylation in a cohort of primary breast cancers but not in normal breast samples, suggesting a putative role for MBD2 in breast cancer.

Original languageEnglish
Article numbere99603
JournalPLoS ONE
Volume9
Issue number6
DOIs
Publication statusPublished - 13 Jun 2014
Externally publishedYes

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