TY - JOUR
T1 - Genome-Wide Location of the Coactivator Mediator
T2 - Binding without Activation and Transient Cdk8 Interaction on DNA
AU - Andrau, Jean Christophe
AU - van de Pasch, Loes
AU - Lijnzaad, Philip
AU - Bijma, Theo
AU - Koerkamp, Marian Groot
AU - van de Peppel, Jeroen
AU - Werner, Michel
AU - Holstege, Frank C.P.
N1 - Funding Information:
We are grateful to M. Ptashne for providing anti-Med15 antibody, to E. van Beek for assistance, M. Timmers for discussions, and X. Gidrol for providing us with the arrays. This work was supported by the following grants from the Netherlands Organization for Scientific Research (NWO): 90101238, 016026009, 80547080, and 05071002.
PY - 2006/4/21
Y1 - 2006/4/21
N2 - Mediator is a general coactivator of RNA polymerase II (Pol II) transcription. Genomic location analyses of different Mediator subunits indicate a uniformly composed core complex upstream of active genes but unexpectedly also upstream of inactive genes and on the coding regions of some highly active genes. The repressive Cdk8 submodule is associated with core Mediator at all sites but with a lower degree of occupancy, indicating transient interaction, regardless of promoter activity. This suggests gene-specific regulation of Cdk8 activity, rather than regulated Cdk8 recruitment. Mediator presence is not necessarily linked to transcription. This goes beyond Cdk8-repressed genes, indicating that Mediator can mark some regulatory regions ahead of additional signals. Overlap with intergenic Pol II location in stationary phase points to a role as a binding platform for inactive Pol II during quiescence. These results shed light on Cdk8 repression, suggest additional roles for Mediator, and query models of recruitment-coupled regulation.
AB - Mediator is a general coactivator of RNA polymerase II (Pol II) transcription. Genomic location analyses of different Mediator subunits indicate a uniformly composed core complex upstream of active genes but unexpectedly also upstream of inactive genes and on the coding regions of some highly active genes. The repressive Cdk8 submodule is associated with core Mediator at all sites but with a lower degree of occupancy, indicating transient interaction, regardless of promoter activity. This suggests gene-specific regulation of Cdk8 activity, rather than regulated Cdk8 recruitment. Mediator presence is not necessarily linked to transcription. This goes beyond Cdk8-repressed genes, indicating that Mediator can mark some regulatory regions ahead of additional signals. Overlap with intergenic Pol II location in stationary phase points to a role as a binding platform for inactive Pol II during quiescence. These results shed light on Cdk8 repression, suggest additional roles for Mediator, and query models of recruitment-coupled regulation.
KW - DNA
UR - http://www.scopus.com/inward/record.url?scp=33646023157&partnerID=8YFLogxK
U2 - 10.1016/j.molcel.2006.03.023
DO - 10.1016/j.molcel.2006.03.023
M3 - Article
C2 - 16630888
AN - SCOPUS:33646023157
SN - 1097-2765
VL - 22
SP - 179
EP - 192
JO - Molecular Cell
JF - Molecular Cell
IS - 2
ER -