Genomic characterization of the human DNA excision repair gene ERCC-1

Marcel van Duin, Marcel H.M. Koken, Jacolien van Den Tol, Peter Ten Dijke, Hanny Odijk, Andries Westerveld, Dirk Bootsma, Jan H.J. Hoeijmakers

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72 Citations (Scopus)

Abstract

In this report the genomic characterization of the human excision repair gene ERCC-1 is presented. The gene consists of 10 exons spread over approximately 15 kb. By means of a transfection assay the ERCC-1 promoter was confined to a region of ± 170 bp upstream of the transcriptional start site. Classical promoter elements like CAAT, TATA and GC-boxes are absent from this region. Furthermore, ERCC-1 transcription is not UV-inducible. A possible explanation is provided for the previously reported alternative splicing of exon VIII. Analysis of ERCC-1 cDNA clones revealed the occurrence of differential polyadenylation which gives ERCC-1 transcripts of 3.4 and 3.8 kb in addition to the major 1.1 kb mRNA. Apparent evolutionary conservation of differential polyadenylation of ERCC-1 transcripts suggests a possible role for this mode of RNA processing in the ERCC-1 repair function.

Original languageEnglish
Pages (from-to)9195-9214
Number of pages20
JournalNucleic Acids Research
Volume15
Issue number22
DOIs
Publication statusPublished - 25 Nov 1987
Externally publishedYes

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