Immunocytochemical detection of hENT1 and hCNT1 in normal tissues, lung cancer cell lines, and NSCLC patient samples

I. Hubeek; E. Giovannetti; A. J.F. Broekhuizen; M. Pastor-Anglada; G. J.L. Kaspers; G. J. Peters

doi:10.1080/15257770802145942

Immunocytochemical detection of hENT1 and hCNT1 in normal tissues, lung cancer cell lines, and NSCLC patient samples

I. Hubeek, E. Giovannetti, A. J.F. Broekhuizen, M. Pastor-Anglada, G. J.L. Kaspers, G. J. Peters

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

Nucleoside transporters are essential for the cellular entry, efficacy, and cytotoxicity of several clinically important deoxynucleoside analogs (e.g., cytarabine and gemcitabine). We used immunohistochemistry to determine protein expression levels of the nucleoside transporters hENT1 and hCNT1 in NSCLC cell lines, NSCLC patient samples, and a variety of normal tissues. All 4 NSCLC cell lines expressed high to very high levels of both hENT1 and hCNT1. In NSCLC and normal tissues expression of hENT1 and hCNT1 ranged from completely negative to high. Immunohistochemistry might be a useful tool to predict response to deoxynucleoside analogs in malignancies treated with these drugs.

Original language	English
Pages (from-to)	787-793
Number of pages	7
Journal	Nucleosides, Nucleotides and Nucleic Acids
Volume	27
Issue number	6-7
DOIs	https://doi.org/10.1080/15257770802145942
Publication status	Published - Jun 2008
Externally published	Yes

Keywords

Antimetabolite cytotoxic drugs
Deoxynucleoside analogs
Human concentrative nucleoside transporters (hCNTs)
Human equilibrative nucleoside transporters (hENTs)
Immunocytochemical staining

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10.1080/15257770802145942

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title = "Immunocytochemical detection of hENT1 and hCNT1 in normal tissues, lung cancer cell lines, and NSCLC patient samples",

abstract = "Nucleoside transporters are essential for the cellular entry, efficacy, and cytotoxicity of several clinically important deoxynucleoside analogs (e.g., cytarabine and gemcitabine). We used immunohistochemistry to determine protein expression levels of the nucleoside transporters hENT1 and hCNT1 in NSCLC cell lines, NSCLC patient samples, and a variety of normal tissues. All 4 NSCLC cell lines expressed high to very high levels of both hENT1 and hCNT1. In NSCLC and normal tissues expression of hENT1 and hCNT1 ranged from completely negative to high. Immunohistochemistry might be a useful tool to predict response to deoxynucleoside analogs in malignancies treated with these drugs.",

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T1 - Immunocytochemical detection of hENT1 and hCNT1 in normal tissues, lung cancer cell lines, and NSCLC patient samples

AU - Hubeek, I.

AU - Giovannetti, E.

AU - Broekhuizen, A. J.F.

AU - Pastor-Anglada, M.

AU - Kaspers, G. J.L.

AU - Peters, G. J.

PY - 2008/6

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AB - Nucleoside transporters are essential for the cellular entry, efficacy, and cytotoxicity of several clinically important deoxynucleoside analogs (e.g., cytarabine and gemcitabine). We used immunohistochemistry to determine protein expression levels of the nucleoside transporters hENT1 and hCNT1 in NSCLC cell lines, NSCLC patient samples, and a variety of normal tissues. All 4 NSCLC cell lines expressed high to very high levels of both hENT1 and hCNT1. In NSCLC and normal tissues expression of hENT1 and hCNT1 ranged from completely negative to high. Immunohistochemistry might be a useful tool to predict response to deoxynucleoside analogs in malignancies treated with these drugs.

KW - Antimetabolite cytotoxic drugs

KW - Deoxynucleoside analogs

KW - Human concentrative nucleoside transporters (hCNTs)

KW - Human equilibrative nucleoside transporters (hENTs)

KW - Immunocytochemical staining

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Immunocytochemical detection of hENT1 and hCNT1 in normal tissues, lung cancer cell lines, and NSCLC patient samples

Abstract

Keywords

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