In planta analysis of the Agrobacterium tumefaciens T-cyt gene promoter: identification of an upstream region essential for promoter activity in leaf, stem and root cells of transgenic tobacco

Saskia T. Neuteboom; Esther Hulleman; Rob A. Schilperoort; J. Harry Hoge

doi:10.1007/BF00027379

In planta analysis of the Agrobacterium tumefaciens T-cyt gene promoter: identification of an upstream region essential for promoter activity in leaf, stem and root cells of transgenic tobacco

Saskia T. Neuteboom, Esther Hulleman, Rob A. Schilperoort, J. Harry Hoge

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

The promoter region of the Agrobacterium tumefaciens T-cyt gene was fused to a beta-glucuronidase (gusA) reporter gene and introduced into tobacco plants. Detection of gusA expression in transgenic F1 progeny revealed that the T-cyt promoter is active in many, if not all, cell types in leaves, stems and roots of fully developed plants. Developmental stage-dependent promoter activity was observed in seedlings. Analysis of 5'-deleted promoter fragments showed that sequences located between positions -185 and -139 with respect to the T-cyt translational start codon are essential for T-cyt promoter activity in transfected tobacco protoplasts as well as in transformed tobacco plants.

Original language	English
Pages (from-to)	923-929
Journal	Plant Molecular Biology
Volume	22
Issue number	5
DOIs	https://doi.org/10.1007/BF00027379
Publication status	Published - Aug 1993
Externally published	Yes

Access to Document

10.1007/BF00027379

Cite this

@article{1d6e1224ecc84b369839b2f61e09b703,

title = "In planta analysis of the Agrobacterium tumefaciens T-cyt gene promoter: identification of an upstream region essential for promoter activity in leaf, stem and root cells of transgenic tobacco",

abstract = "The promoter region of the Agrobacterium tumefaciens T-cyt gene was fused to a beta-glucuronidase (gusA) reporter gene and introduced into tobacco plants. Detection of gusA expression in transgenic F1 progeny revealed that the T-cyt promoter is active in many, if not all, cell types in leaves, stems and roots of fully developed plants. Developmental stage-dependent promoter activity was observed in seedlings. Analysis of 5'-deleted promoter fragments showed that sequences located between positions -185 and -139 with respect to the T-cyt translational start codon are essential for T-cyt promoter activity in transfected tobacco protoplasts as well as in transformed tobacco plants.",

author = "Neuteboom, {Saskia T.} and Esther Hulleman and Schilperoort, {Rob A.} and Hoge, {J. Harry}",

year = "1993",

month = aug,

doi = "10.1007/BF00027379",

language = "English",

volume = "22",

pages = "923--929",

journal = "Plant Molecular Biology",

issn = "0167-4412",

publisher = "Springer Science and Business Media B.V.",

number = "5",

}

In planta analysis of the Agrobacterium tumefaciens T-cyt gene promoter: identification of an upstream region essential for promoter activity in leaf, stem and root cells of transgenic tobacco. / Neuteboom, Saskia T.; Hulleman, Esther; Schilperoort, Rob A. et al.
In: Plant Molecular Biology, Vol. 22, No. 5, 08.1993, p. 923-929.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - In planta analysis of the Agrobacterium tumefaciens T-cyt gene promoter: identification of an upstream region essential for promoter activity in leaf, stem and root cells of transgenic tobacco

AU - Neuteboom, Saskia T.

AU - Hulleman, Esther

AU - Schilperoort, Rob A.

AU - Hoge, J. Harry

PY - 1993/8

Y1 - 1993/8

N2 - The promoter region of the Agrobacterium tumefaciens T-cyt gene was fused to a beta-glucuronidase (gusA) reporter gene and introduced into tobacco plants. Detection of gusA expression in transgenic F1 progeny revealed that the T-cyt promoter is active in many, if not all, cell types in leaves, stems and roots of fully developed plants. Developmental stage-dependent promoter activity was observed in seedlings. Analysis of 5'-deleted promoter fragments showed that sequences located between positions -185 and -139 with respect to the T-cyt translational start codon are essential for T-cyt promoter activity in transfected tobacco protoplasts as well as in transformed tobacco plants.

AB - The promoter region of the Agrobacterium tumefaciens T-cyt gene was fused to a beta-glucuronidase (gusA) reporter gene and introduced into tobacco plants. Detection of gusA expression in transgenic F1 progeny revealed that the T-cyt promoter is active in many, if not all, cell types in leaves, stems and roots of fully developed plants. Developmental stage-dependent promoter activity was observed in seedlings. Analysis of 5'-deleted promoter fragments showed that sequences located between positions -185 and -139 with respect to the T-cyt translational start codon are essential for T-cyt promoter activity in transfected tobacco protoplasts as well as in transformed tobacco plants.

U2 - 10.1007/BF00027379

DO - 10.1007/BF00027379

M3 - Article

SN - 0167-4412

VL - 22

SP - 923

EP - 929

JO - Plant Molecular Biology

JF - Plant Molecular Biology

IS - 5

ER -

In planta analysis of the Agrobacterium tumefaciens T-cyt gene promoter: identification of an upstream region essential for promoter activity in leaf, stem and root cells of transgenic tobacco

Abstract

Access to Document

Fingerprint

Cite this