In situ hybridization to identify gut stem cells

Alex Gregorieff; Hans Clevers

doi:10.1002/9780470151808.sc02f01s34

In situ hybridization to identify gut stem cells

Alex Gregorieff
, Hans Clevers

Research output: Contribution to journal › Article › peer-review

34 Citations (Scopus)

Abstract

In recent years, considerable effort has been directed toward identifying the repertoire of genes specifically expressed in adult stem cells. In this unit, we describe an in situ hybridization protocol adapted for the analysis of gene expression in the intestinal mucosa. This methodology allows researchers to quickly visualize the expression profile of putative stem cell markers with a high degree of sensitivity and resolution.

Original language	English
Pages (from-to)	2F.1.1-2F.1.10
Journal	Current Protocols in Stem Cell Biology
Volume	2015
DOIs	https://doi.org/10.1002/9780470151808.sc02f01s34 https://doi.org/10.1002/9780470151808.sc02f01s12
Publication status	Published - 2015
Externally published	Yes

Keywords

(ISH) in situ hybridization
Digoxigenin RNA probes
Formalin-fixed paraffin-embedded
Intestinal sections

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title = "In situ hybridization to identify gut stem cells",

abstract = "In recent years, considerable effort has been directed toward identifying the repertoire of genes specifically expressed in adult stem cells. In this unit, we describe an in situ hybridization protocol adapted for the analysis of gene expression in the intestinal mucosa. This methodology allows researchers to quickly visualize the expression profile of putative stem cell markers with a high degree of sensitivity and resolution.",

keywords = "(ISH) in situ hybridization, Digoxigenin RNA probes, Formalin-fixed paraffin-embedded, Intestinal sections",

author = "Alex Gregorieff and Hans Clevers",

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year = "2015",

doi = "10.1002/9780470151808.sc02f01s34",

language = "English",

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T1 - In situ hybridization to identify gut stem cells

AU - Gregorieff, Alex

AU - Clevers, Hans

PY - 2015

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AB - In recent years, considerable effort has been directed toward identifying the repertoire of genes specifically expressed in adult stem cells. In this unit, we describe an in situ hybridization protocol adapted for the analysis of gene expression in the intestinal mucosa. This methodology allows researchers to quickly visualize the expression profile of putative stem cell markers with a high degree of sensitivity and resolution.

KW - (ISH) in situ hybridization

KW - Digoxigenin RNA probes

KW - Formalin-fixed paraffin-embedded

KW - Intestinal sections

UR - https://www.scopus.com/pages/publications/75949124381

U2 - 10.1002/9780470151808.sc02f01s34

DO - 10.1002/9780470151808.sc02f01s34

M3 - Article

C2 - 26237569

AN - SCOPUS:75949124381

SN - 1941-7322

VL - 2015

SP - 2F.1.1-2F.1.10

JO - Current Protocols in Stem Cell Biology

JF - Current Protocols in Stem Cell Biology

ER -

In situ hybridization to identify gut stem cells

Abstract

Keywords

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