Instability of human TATA-binding protein CAG triplet repeats during amplification by PCR

Frank C.P. Holstege; Peter C. van der Vliet; H. Th Marc Timmers

doi:10.1016/0167-4781(94)90260-7

Instability of human TATA-binding protein CAG triplet repeats during amplification by PCR

Frank C.P. Holstege
, Peter C. van der Vliet
, H. Th Marc Timmers

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

Polymerase chain reaction (PCR) of a TATA-binding protein cDNA that contains CAG triplet repeats results in heterogeneous products. This is caused by a variable loss in the number of CAG triplets. Sequence analysis of PCR products suggests that instability increases with repeat length.

Original language	English
Pages (from-to)	157-159
Number of pages	3
Journal	BBA - Gene Structure and Expression
Volume	1219
Issue number	1
DOIs	https://doi.org/10.1016/0167-4781(94)90260-7
Publication status	Published - 13 Sept 1994
Externally published	Yes

Keywords

Dynamic mutation
PCR
PCR instability
TATA-binding protein
Triplet repeat expansion

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10.1016/0167-4781(94)90260-7

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@article{44fcfefdb0a4437d9076f74edbc97800,

title = "Instability of human TATA-binding protein CAG triplet repeats during amplification by PCR",

abstract = "Polymerase chain reaction (PCR) of a TATA-binding protein cDNA that contains CAG triplet repeats results in heterogeneous products. This is caused by a variable loss in the number of CAG triplets. Sequence analysis of PCR products suggests that instability increases with repeat length.",

keywords = "Dynamic mutation, PCR, PCR instability, TATA-binding protein, Triplet repeat expansion",

author = "Holstege, \{Frank C.P.\} and \{van der Vliet\}, \{Peter C.\} and Timmers, \{H. Th Marc\}",

note = "Funding Information: We wish to thank Elly Holthuizen for oligonucleotide synthesis and Marian Walhout for critically reviewing this manuscript. The research of H.Th.M.T. was supported by a fellowship from the Royal Netherlands Academy of Arts and Sciences. This work was also supported in part by the Netherlands Foundation for Chemical Research (SON) and with financial support from the Netherlands Organization for Scientific Research (NWO).",

year = "1994",

month = sep,

day = "13",

doi = "10.1016/0167-4781(94)90260-7",

language = "English",

volume = "1219",

pages = "157--159",

journal = "BBA - Gene Structure and Expression",

issn = "0167-4781",

publisher = "Elsevier B.V.",

number = "1",

}

TY - JOUR

T1 - Instability of human TATA-binding protein CAG triplet repeats during amplification by PCR

AU - Holstege, Frank C.P.

AU - van der Vliet, Peter C.

AU - Timmers, H. Th Marc

N1 - Funding Information: We wish to thank Elly Holthuizen for oligonucleotide synthesis and Marian Walhout for critically reviewing this manuscript. The research of H.Th.M.T. was supported by a fellowship from the Royal Netherlands Academy of Arts and Sciences. This work was also supported in part by the Netherlands Foundation for Chemical Research (SON) and with financial support from the Netherlands Organization for Scientific Research (NWO).

PY - 1994/9/13

Y1 - 1994/9/13

N2 - Polymerase chain reaction (PCR) of a TATA-binding protein cDNA that contains CAG triplet repeats results in heterogeneous products. This is caused by a variable loss in the number of CAG triplets. Sequence analysis of PCR products suggests that instability increases with repeat length.

AB - Polymerase chain reaction (PCR) of a TATA-binding protein cDNA that contains CAG triplet repeats results in heterogeneous products. This is caused by a variable loss in the number of CAG triplets. Sequence analysis of PCR products suggests that instability increases with repeat length.

KW - Dynamic mutation

KW - PCR

KW - PCR instability

KW - TATA-binding protein

KW - Triplet repeat expansion

UR - https://www.scopus.com/pages/publications/0028038270

U2 - 10.1016/0167-4781(94)90260-7

DO - 10.1016/0167-4781(94)90260-7

M3 - Article

C2 - 8086455

AN - SCOPUS:0028038270

SN - 0167-4781

VL - 1219

SP - 157

EP - 159

JO - BBA - Gene Structure and Expression

JF - BBA - Gene Structure and Expression

IS - 1

ER -

Instability of human TATA-binding protein CAG triplet repeats during amplification by PCR

Abstract

Keywords

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