Interaction of hHR23 with S5a. The ubiquitin-like domain of hHR23 mediates interaction with S5a subunit of 26 S proteasome

Hideki Hiyama, Masayuki Yokoi, Chikahide Masutani, Kaoru Sugasawa, Takafumi Maekawa, Keiji Tanaka, Jan H.J. Hoeijmakers, Fumio Hanaoka

Research output: Contribution to journalArticlepeer-review

232 Citations (Scopus)


hHR23B is one of two human homologs of the Saccharomyces cerevisiae nucleotide excision repair (NER) gene product RAD23 and a component of a protein complex that specifically complements the NER defect of xeroderma pigmentosum group C (XP-C) cell extracts in vitro. Although a small proportion of hHR23B is tightly complexed with the XP-C responsible gene product, XPC protein, a vast majority exists as an XPC-free form, indicating that hHR23B has additional functions other than NER in vivo. Here we demonstrate that the human NER factor hHR23B as well as another human homolog of RAD23, hHR23A, interact specifically with S5a, a subunit of the human 26 S proteasome using the yeast two-hybrid system. Furthermore, hHR23 proteins were detected with S5a at the position where 26 S proteasome sediments in glycerol gradient centrifugation of HeLa S100 extracts. Intriguingly, hHR23B showed the inhibitory effect on the degradation of 125I-lysozyme in the rabbit reticulocyte lysate, hHR23 proteins thus appear to associate with 26 S proteasome in vivo. From co-precipitation experiments using several series of deletion mutants, we defined the domains in hHR23B and S5a that mediate this interaction. From these results, we propose that part of hHR23 proteins are involved in the proteolytic pathway in cells.

Original languageEnglish
Pages (from-to)28019-28025
Number of pages7
JournalJournal of Biological Chemistry
Issue number39
Publication statusPublished - 24 Sept 1999
Externally publishedYes


Dive into the research topics of 'Interaction of hHR23 with S5a. The ubiquitin-like domain of hHR23 mediates interaction with S5a subunit of 26 S proteasome'. Together they form a unique fingerprint.

Cite this