Kinetoplast DNA in the insect trypanosomes Crithidia luciliae and Crithidia fasciculata. III. Heteroduplex analysis of the C. luciliae minicircles

J. H.J. Hoeijmakers; P. J. Welters; G. J. Brakenhoff; P. Borst

doi:10.1016/0147-619X(82)90003-8

Kinetoplast DNA in the insect trypanosomes Crithidia luciliae and Crithidia fasciculata. III. Heteroduplex analysis of the C. luciliae minicircles

J. H.J. Hoeijmakers
, P. J. Welters
, G. J. Brakenhoff
, P. Borst

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

We have investigated the nature of the sequence heterogeneity of the minicircles of Crithidia luciliae kinetoplast DNA by EM heteroduplex analysis of minicircles cleaved with endonuclease HindIII. Approximately 40% of the minicircles showed-after denaturation and reannealing-structures indicative of sequence rearrangements: the majority contained heteroduplex "eyes" interpreted as due to inversions; about 10% of the heteroduplexes yielded structures interpreted as due to translocations and a similar fraction showed insertions and deletions. The category of "eyed" molecules was analyzed in detail: four minicircle segments were found that displayed a high incidence of such eyes indicating that the rearrangements were not scattered at random over the minicircles. Moreover, since also "eyes" were found overlapping two or three of the four regions, we postulate that these segments are capable of recombining with each other. We conclude that specific segmental rearrangements form the main basis of the minicircle sequence heterogeneity in Crithidia.

Original language	English
Pages (from-to)	221-229
Number of pages	9
Journal	Plasmid
Volume	7
Issue number	3
DOIs	https://doi.org/10.1016/0147-619X(82)90003-8
Publication status	Published - May 1982
Externally published	Yes

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@article{59cacc50503d468aa5e053e2cb234230,

title = "Kinetoplast DNA in the insect trypanosomes Crithidia luciliae and Crithidia fasciculata. III. Heteroduplex analysis of the C. luciliae minicircles",

abstract = "We have investigated the nature of the sequence heterogeneity of the minicircles of Crithidia luciliae kinetoplast DNA by EM heteroduplex analysis of minicircles cleaved with endonuclease HindIII. Approximately 40\% of the minicircles showed-after denaturation and reannealing-structures indicative of sequence rearrangements: the majority contained heteroduplex {"}eyes{"} interpreted as due to inversions; about 10\% of the heteroduplexes yielded structures interpreted as due to translocations and a similar fraction showed insertions and deletions. The category of {"}eyed{"} molecules was analyzed in detail: four minicircle segments were found that displayed a high incidence of such eyes indicating that the rearrangements were not scattered at random over the minicircles. Moreover, since also {"}eyes{"} were found overlapping two or three of the four regions, we postulate that these segments are capable of recombining with each other. We conclude that specific segmental rearrangements form the main basis of the minicircle sequence heterogeneity in Crithidia.",

author = "Hoeijmakers, \{J. H.J.\} and Welters, \{P. J.\} and Brakenhoff, \{G. J.\} and P. Borst",

note = "Funding Information: We are indebtedt o the Staff of the Laboratory of Electron Microscopy( Head Prof. N. Nanninga) of the University of Amsterdamf or their generousp rovision of facilities.W e thank Miss J. Van den Burg and Miss M. Van den Berg for expert technicala ssistanceM, rs. Rita Boucke for typing of the manuscript,a nd Mr. T. Van OS and Mr. J. Woonsf or helpw ith the photographs. This work was supportedi n part by a grant from The Netherlands Foundation for Fundamental Biological Research( BION) with financial aid from The Orga-nizationf or the Advancemenot f Pure Research( ZWO).",

year = "1982",

month = may,

doi = "10.1016/0147-619X(82)90003-8",

language = "English",

volume = "7",

pages = "221--229",

journal = "Plasmid",

issn = "0147-619X",

publisher = "Academic Press Inc.",

number = "3",

}

TY - JOUR

T1 - Kinetoplast DNA in the insect trypanosomes Crithidia luciliae and Crithidia fasciculata. III. Heteroduplex analysis of the C. luciliae minicircles

AU - Hoeijmakers, J. H.J.

AU - Welters, P. J.

AU - Brakenhoff, G. J.

AU - Borst, P.

N1 - Funding Information: We are indebtedt o the Staff of the Laboratory of Electron Microscopy( Head Prof. N. Nanninga) of the University of Amsterdamf or their generousp rovision of facilities.W e thank Miss J. Van den Burg and Miss M. Van den Berg for expert technicala ssistanceM, rs. Rita Boucke for typing of the manuscript,a nd Mr. T. Van OS and Mr. J. Woonsf or helpw ith the photographs. This work was supportedi n part by a grant from The Netherlands Foundation for Fundamental Biological Research( BION) with financial aid from The Orga-nizationf or the Advancemenot f Pure Research( ZWO).

PY - 1982/5

Y1 - 1982/5

N2 - We have investigated the nature of the sequence heterogeneity of the minicircles of Crithidia luciliae kinetoplast DNA by EM heteroduplex analysis of minicircles cleaved with endonuclease HindIII. Approximately 40% of the minicircles showed-after denaturation and reannealing-structures indicative of sequence rearrangements: the majority contained heteroduplex "eyes" interpreted as due to inversions; about 10% of the heteroduplexes yielded structures interpreted as due to translocations and a similar fraction showed insertions and deletions. The category of "eyed" molecules was analyzed in detail: four minicircle segments were found that displayed a high incidence of such eyes indicating that the rearrangements were not scattered at random over the minicircles. Moreover, since also "eyes" were found overlapping two or three of the four regions, we postulate that these segments are capable of recombining with each other. We conclude that specific segmental rearrangements form the main basis of the minicircle sequence heterogeneity in Crithidia.

AB - We have investigated the nature of the sequence heterogeneity of the minicircles of Crithidia luciliae kinetoplast DNA by EM heteroduplex analysis of minicircles cleaved with endonuclease HindIII. Approximately 40% of the minicircles showed-after denaturation and reannealing-structures indicative of sequence rearrangements: the majority contained heteroduplex "eyes" interpreted as due to inversions; about 10% of the heteroduplexes yielded structures interpreted as due to translocations and a similar fraction showed insertions and deletions. The category of "eyed" molecules was analyzed in detail: four minicircle segments were found that displayed a high incidence of such eyes indicating that the rearrangements were not scattered at random over the minicircles. Moreover, since also "eyes" were found overlapping two or three of the four regions, we postulate that these segments are capable of recombining with each other. We conclude that specific segmental rearrangements form the main basis of the minicircle sequence heterogeneity in Crithidia.

UR - https://www.scopus.com/pages/publications/0019911576

U2 - 10.1016/0147-619X(82)90003-8

DO - 10.1016/0147-619X(82)90003-8

M3 - Article

C2 - 7100301

AN - SCOPUS:0019911576

SN - 0147-619X

VL - 7

SP - 221

EP - 229

JO - Plasmid

JF - Plasmid

IS - 3

ER -

Kinetoplast DNA in the insect trypanosomes Crithidia luciliae and Crithidia fasciculata. III. Heteroduplex analysis of the C. luciliae minicircles

Abstract

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