Lack of Bcl10 mutations in testicular germ cell tumours and derived cell lines

E M van Schothorst; S Mohkamsing; R J van Gurp; J W Oosterhuis; P T van der Saag; L H Looijenga

doi:10.1038/sj.bjc.6690563

Lack of Bcl10 mutations in testicular germ cell tumours and derived cell lines

E M van Schothorst
, S Mohkamsing
, R J van Gurp
, J W Oosterhuis
, P T van der Saag
, L H Looijenga

Research output: Contribution to journal › Article › peer-review

23 Citations (Scopus)

Abstract

Aberrations within Bcl10, a gene involved in execution of apoptosis, has most recently been found in a variety of cancers, including cell lines of testicular germ cell tumours of adolescents and adults (TGCTs). To study this in more detail, we screened exons 2 and 3 of this gene for mutations in a larger series of cell lines as well as primary TGCTs by single-strand conformation polymorphism and endonuclease restriction analysis. Because no aberrations were detected, we conclude that inactivation of Bcl10 by mutation is at least far less important in the development of TGCTs than proposed.

Original language	English
Pages (from-to)	1571-4
Number of pages	4
Journal	British journal of cancer
Volume	80
Issue number	10
DOIs	https://doi.org/10.1038/sj.bjc.6690563
Publication status	Published - Jul 1999
Externally published	Yes

Keywords

Adaptor Proteins, Signal Transducing
Adolescent
Adult
B-Cell CLL-Lymphoma 10 Protein
Base Sequence
DNA, Neoplasm
Exons
Germinoma/genetics
Humans
Male
Molecular Sequence Data
Neoplasm Proteins/genetics
Polymorphism, Single-Stranded Conformational
Testicular Neoplasms/genetics
Tumor Cells, Cultured

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10.1038/sj.bjc.6690563

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@article{e19a78078a5a437aa1f4d10a3ed167af,

title = "Lack of Bcl10 mutations in testicular germ cell tumours and derived cell lines",

abstract = "Aberrations within Bcl10, a gene involved in execution of apoptosis, has most recently been found in a variety of cancers, including cell lines of testicular germ cell tumours of adolescents and adults (TGCTs). To study this in more detail, we screened exons 2 and 3 of this gene for mutations in a larger series of cell lines as well as primary TGCTs by single-strand conformation polymorphism and endonuclease restriction analysis. Because no aberrations were detected, we conclude that inactivation of Bcl10 by mutation is at least far less important in the development of TGCTs than proposed.",

keywords = "Adaptor Proteins, Signal Transducing, Adolescent, Adult, B-Cell CLL-Lymphoma 10 Protein, Base Sequence, DNA, Neoplasm, Exons, Germinoma/genetics, Humans, Male, Molecular Sequence Data, Neoplasm Proteins/genetics, Polymorphism, Single-Stranded Conformational, Testicular Neoplasms/genetics, Tumor Cells, Cultured",

author = "\{van Schothorst\}, \{E M\} and S Mohkamsing and \{van Gurp\}, \{R J\} and Oosterhuis, \{J W\} and \{van der Saag\}, \{P T\} and Looijenga, \{L H\}",

note = "Funding Information: This project is financially supported by the Dutch Cancer Society (JWO and LL, and grant 97 1516 for EMvS and SM). We kindly thank Dr MA Versnel, Department of Immunology, Erasmus University Rotterdam, The Netherlands for providing the mesothelioma cell line.",

year = "1999",

month = jul,

doi = "10.1038/sj.bjc.6690563",

language = "English",

volume = "80",

pages = "1571--4",

journal = "British journal of cancer",

issn = "0007-0920",

publisher = "Springer Nature",

number = "10",

}

TY - JOUR

T1 - Lack of Bcl10 mutations in testicular germ cell tumours and derived cell lines

AU - van Schothorst, E M

AU - Mohkamsing, S

AU - van Gurp, R J

AU - Oosterhuis, J W

AU - van der Saag, P T

AU - Looijenga, L H

N1 - Funding Information: This project is financially supported by the Dutch Cancer Society (JWO and LL, and grant 97 1516 for EMvS and SM). We kindly thank Dr MA Versnel, Department of Immunology, Erasmus University Rotterdam, The Netherlands for providing the mesothelioma cell line.

PY - 1999/7

Y1 - 1999/7

N2 - Aberrations within Bcl10, a gene involved in execution of apoptosis, has most recently been found in a variety of cancers, including cell lines of testicular germ cell tumours of adolescents and adults (TGCTs). To study this in more detail, we screened exons 2 and 3 of this gene for mutations in a larger series of cell lines as well as primary TGCTs by single-strand conformation polymorphism and endonuclease restriction analysis. Because no aberrations were detected, we conclude that inactivation of Bcl10 by mutation is at least far less important in the development of TGCTs than proposed.

AB - Aberrations within Bcl10, a gene involved in execution of apoptosis, has most recently been found in a variety of cancers, including cell lines of testicular germ cell tumours of adolescents and adults (TGCTs). To study this in more detail, we screened exons 2 and 3 of this gene for mutations in a larger series of cell lines as well as primary TGCTs by single-strand conformation polymorphism and endonuclease restriction analysis. Because no aberrations were detected, we conclude that inactivation of Bcl10 by mutation is at least far less important in the development of TGCTs than proposed.

KW - Adaptor Proteins, Signal Transducing

KW - Adolescent

KW - Adult

KW - B-Cell CLL-Lymphoma 10 Protein

KW - Base Sequence

KW - DNA, Neoplasm

KW - Exons

KW - Germinoma/genetics

KW - Humans

KW - Male

KW - Molecular Sequence Data

KW - Neoplasm Proteins/genetics

KW - Polymorphism, Single-Stranded Conformational

KW - Testicular Neoplasms/genetics

KW - Tumor Cells, Cultured

UR - https://www.scopus.com/pages/publications/0033029861

U2 - 10.1038/sj.bjc.6690563

DO - 10.1038/sj.bjc.6690563

M3 - Article

C2 - 10408400

SN - 0007-0920

VL - 80

SP - 1571

EP - 1574

JO - British journal of cancer

JF - British journal of cancer

IS - 10

ER -

Lack of Bcl10 mutations in testicular germ cell tumours and derived cell lines

Abstract

Keywords

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