Localization and polymorphism of a chromosome 12-specific alpha satellite DNA sequence

L H Looijenga; V T Smit; J W Wessels; P Mollevanger; J W Oosterhuis; C J Cornelisse; P Devilee

doi:10.1159/000132934

Localization and polymorphism of a chromosome 12-specific alpha satellite DNA sequence

L H Looijenga
, V T Smit
, J W Wessels
, P Mollevanger
, J W Oosterhuis
, C J Cornelisse
, P Devilee

Research output: Contribution to journal › Article › peer-review

72 Citations (Scopus)

Abstract

The isolation and localization of a chromosome 12-specific alpha satellite DNA sequence, p alpha 12H8, is described. This clone contains a complete copy of the 1.4-kb HindIII higher-order repeat present within the alpha satellite array on chromosome 12. The specificity of p alpha 12H8 was demonstrated by in situ hybridization and Southern blot analysis of a somatic cell hybrid mapping panel, both performed under high-stringency conditions. Polymorphic restriction patterns within the alpha satellite array, revealed by the use of the restriction enzymes BglII and EcoRV, were demonstrated to display Mendelian inheritance. These properties make p alpha 12H8 a valuable genetic marker for the centromeric region of chromosome 12.

Original language	English
Pages (from-to)	216-8
Number of pages	3
Journal	Cytogenetics and cell genetics
Volume	53
Issue number	4
DOIs	https://doi.org/10.1159/000132934
Publication status	Published - 1990
Externally published	Yes

Keywords

Centromere
Chromosome Mapping
Chromosomes, Human, Pair 12
DNA Probes
DNA, Satellite/genetics
Humans
Nucleic Acid Hybridization
Polymorphism, Restriction Fragment Length

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10.1159/000132934

Cite this

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title = "Localization and polymorphism of a chromosome 12-specific alpha satellite DNA sequence",

abstract = "The isolation and localization of a chromosome 12-specific alpha satellite DNA sequence, p alpha 12H8, is described. This clone contains a complete copy of the 1.4-kb HindIII higher-order repeat present within the alpha satellite array on chromosome 12. The specificity of p alpha 12H8 was demonstrated by in situ hybridization and Southern blot analysis of a somatic cell hybrid mapping panel, both performed under high-stringency conditions. Polymorphic restriction patterns within the alpha satellite array, revealed by the use of the restriction enzymes BglII and EcoRV, were demonstrated to display Mendelian inheritance. These properties make p alpha 12H8 a valuable genetic marker for the centromeric region of chromosome 12.",

keywords = "Centromere, Chromosome Mapping, Chromosomes, Human, Pair 12, DNA Probes, DNA, Satellite/genetics, Humans, Nucleic Acid Hybridization, Polymorphism, Restriction Fragment Length",

author = "Looijenga, \{L H\} and Smit, \{V T\} and Wessels, \{J W\} and P Mollevanger and Oosterhuis, \{J W\} and Cornelisse, \{C J\} and P Devilee",

year = "1990",

doi = "10.1159/000132934",

language = "English",

volume = "53",

pages = "216--8",

journal = "Cytogenetics and cell genetics",

issn = "0301-0171",

publisher = "S. Karger AG",

number = "4",

}

TY - JOUR

T1 - Localization and polymorphism of a chromosome 12-specific alpha satellite DNA sequence

AU - Looijenga, L H

AU - Smit, V T

AU - Wessels, J W

AU - Mollevanger, P

AU - Oosterhuis, J W

AU - Cornelisse, C J

AU - Devilee, P

PY - 1990

Y1 - 1990

N2 - The isolation and localization of a chromosome 12-specific alpha satellite DNA sequence, p alpha 12H8, is described. This clone contains a complete copy of the 1.4-kb HindIII higher-order repeat present within the alpha satellite array on chromosome 12. The specificity of p alpha 12H8 was demonstrated by in situ hybridization and Southern blot analysis of a somatic cell hybrid mapping panel, both performed under high-stringency conditions. Polymorphic restriction patterns within the alpha satellite array, revealed by the use of the restriction enzymes BglII and EcoRV, were demonstrated to display Mendelian inheritance. These properties make p alpha 12H8 a valuable genetic marker for the centromeric region of chromosome 12.

AB - The isolation and localization of a chromosome 12-specific alpha satellite DNA sequence, p alpha 12H8, is described. This clone contains a complete copy of the 1.4-kb HindIII higher-order repeat present within the alpha satellite array on chromosome 12. The specificity of p alpha 12H8 was demonstrated by in situ hybridization and Southern blot analysis of a somatic cell hybrid mapping panel, both performed under high-stringency conditions. Polymorphic restriction patterns within the alpha satellite array, revealed by the use of the restriction enzymes BglII and EcoRV, were demonstrated to display Mendelian inheritance. These properties make p alpha 12H8 a valuable genetic marker for the centromeric region of chromosome 12.

KW - Centromere

KW - Chromosome Mapping

KW - Chromosomes, Human, Pair 12

KW - DNA Probes

KW - DNA, Satellite/genetics

KW - Humans

KW - Nucleic Acid Hybridization

KW - Polymorphism, Restriction Fragment Length

UR - https://www.scopus.com/pages/publications/0025115707

U2 - 10.1159/000132934

DO - 10.1159/000132934

M3 - Article

C2 - 1976486

SN - 0301-0171

VL - 53

SP - 216

EP - 218

JO - Cytogenetics and cell genetics

JF - Cytogenetics and cell genetics

IS - 4

ER -

Localization and polymorphism of a chromosome 12-specific alpha satellite DNA sequence

Abstract

Keywords

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