TY - JOUR
T1 - Localization of the xeroderma pigmentosum group B-correcting gene ERCC3 to human chromosome 2q21
AU - Weeda, Geert
AU - Wiegant, Joop
AU - van der Ploeg, Mels
AU - Geurts van Kessel, Ad H.M.
AU - van der Eb, Alex J.
AU - Hoeijmakers, Jan H.J.
N1 - Funding Information:
We thank Professor D. Bootama for his stimulating interest, continuous support, and critical reading of this manuscript; E. M. E. Smit and A. Hagemeyer for the in situ hybridization periments using 3H-labeled probes; and M. Kuit for photography. This work was supported financially by the Netherlands Organization for Advancement of Pure Research (NWO) through the Foundation of Medical Scientific Research (Contract 900-501-091) and by the Commission of the European Community (Contract B16-141-NL).
PY - 1991/8
Y1 - 1991/8
N2 - The human excision-repair gene ERCC3 was cloned after DNA-mediated gene transfer to the uv-sensitive Chinese hamster ovary mutant cell line 27-1, a member of complementation group 3 of the excision-defective rodent cell lines. The ERCC3 gene specifically corrects the DNA repair defect of xeroderma pigmentosum (XP) complementation group B, which displays the clinical symptoms of XP as well as of another rare excision-repair disorder, Cockayne syndrome. The gene encodes a presumed DNA and chromatin binding helicase, involved in early steps of the excision-repair pathway. ERCC3 was previously assigned to human chromosome 2 (L. H. Thompson, A. V. Carrano, K. Sato, E. P. Salazar, B. F. White, S. A. Stewart, J. L. Minkler, and M. J. Siciliano (1987)Somat. Cell Genet. 13: 539-551). Here we report its subchromosomal localization in the q21 region of chromosome 2 via somatic cell hybrids containing a translocated chromosome 2 and in situ hybridization with fluorescently labeled ERCC3 probes.
AB - The human excision-repair gene ERCC3 was cloned after DNA-mediated gene transfer to the uv-sensitive Chinese hamster ovary mutant cell line 27-1, a member of complementation group 3 of the excision-defective rodent cell lines. The ERCC3 gene specifically corrects the DNA repair defect of xeroderma pigmentosum (XP) complementation group B, which displays the clinical symptoms of XP as well as of another rare excision-repair disorder, Cockayne syndrome. The gene encodes a presumed DNA and chromatin binding helicase, involved in early steps of the excision-repair pathway. ERCC3 was previously assigned to human chromosome 2 (L. H. Thompson, A. V. Carrano, K. Sato, E. P. Salazar, B. F. White, S. A. Stewart, J. L. Minkler, and M. J. Siciliano (1987)Somat. Cell Genet. 13: 539-551). Here we report its subchromosomal localization in the q21 region of chromosome 2 via somatic cell hybrids containing a translocated chromosome 2 and in situ hybridization with fluorescently labeled ERCC3 probes.
UR - http://www.scopus.com/inward/record.url?scp=0025772861&partnerID=8YFLogxK
U2 - 10.1016/0888-7543(91)90195-K
DO - 10.1016/0888-7543(91)90195-K
M3 - Article
C2 - 1916809
AN - SCOPUS:0025772861
SN - 0888-7543
VL - 10
SP - 1035
EP - 1040
JO - Genomics
JF - Genomics
IS - 4
ER -