@inbook{1a045c17f0774ff7a94a7dbe1db852fd,
title = "Long-Term Expansion of Murine Primary Hepatocyte Organoids",
abstract = "The in vitro maintenance and expansion of primary hepatocytes provide immense opportunities for disease modeling and other biological, viral, and toxicological studies, as well as for applications in regenerative medicine such as cell transplantation for the treatment of metabolic liver diseases. Here, we describe a protocol for the isolation and in vitro culture of primary hepatocytes in a three-dimensional extracellular matrix gel. The inflammatory cytokine tumor necrosis factor alpha (TNFα) is crucial for the long-term expansion of mouse hepatocyte organoids by mimicking the regenerative response in vitro. Long-term cultured hepatocyte organoids express high levels of hepatocyte markers upon differentiation.",
keywords = "Culture, Cytokines, Hepatocytes, Liver, Mouse, Organoids, Regeneration, TNFα, Animals, Tumor Necrosis Factor-alpha/metabolism, Hepatocytes/metabolism, Liver/metabolism, Cell Differentiation, Mice, Culture, Cytokines, Hepatocytes, Liver, Mouse, Organoids, Regeneration, TNFα",
author = "Kluiver, {Thomas A.} and Kraaier, {Lianne J.} and Peng, {Weng Chuan}",
note = "{\textcopyright} 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2022",
doi = "10.1007/978-1-0716-2557-6_1",
language = "English",
volume = "2544",
series = "Methods in molecular biology (Clifton, N.J.)",
publisher = "Humana Press Inc.",
pages = "1--13",
booktitle = "Methods in Molecular Biology",
}