Medium-throughput drug-and radiotherapy screening assay using patient-derived organoids

Marrit Putker, Rosemary Millen, René Overmeer, Else Driehuis, Maurice M.J.M. Zandvliet, Hans Clevers, Sylvia F. Boj, Qi Xiang Li

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

Patient-derived organoid (PDO) models allow for long-term expansion and maintenance of primary epithelial cells grown in three dimensions and a near-native state. When derived from resected or biopsied tumor tissue, organoids closely recapitulate in vivo tumor morphology and can be used to study therapy response in vitro. Biobanks of tumor organoids reflect the vast variety of clinical tumors and patients and therefore hold great promise for preclinical and clinical applications. This paper presents a method for medium-throughput drug screening using head and neck squamous cell carcinoma and colorectal adenocarcinoma organoids. This approach can easily be adopted for use with any tissue-derived organoid model, both normal and diseased. Methods are described for in vitro exposure of organoids to chemo-and radiotherapy (either as single-treatment modality or in combination). Cell survival after 5 days of drug exposure is assessed by measuring adenosine triphosphate (ATP) levels. Drug sensitivity is measured by the half-maximal inhibitory concentration (IC50), area under the curve (AUC), and growth rate (GR) metrics. These parameters can provide insight into whether an organoid culture is deemed sensitive or resistant to a particular treatment.

Original languageEnglish
Article numbere62495
JournalJournal of Visualized Experiments
Volume2021
Issue number170
DOIs
Publication statusPublished - 2021
Externally publishedYes

Fingerprint

Dive into the research topics of 'Medium-throughput drug-and radiotherapy screening assay using patient-derived organoids'. Together they form a unique fingerprint.

Cite this