Micronuclei-based model system reveals functional consequences of chromothripsis in human cells

Maja Kneissig, Kristina Keuper, Mirjam S. De Pagter, Markus J. Van Roosmalen, Jana Martin, Hannah Otto, Verena Passerini, Aline Campos Sparr, Ivo Renkens, Fenna Kropveld, Anand Vasudevan, Jason Sheltzer, Wigard P. Kloosterman, Zuzana Storchová

Research output: Contribution to journalArticlepeer-review

61 Citations (Scopus)

Abstract

Cancer cells often harbor chromosomes in abnormal numbers and with aberrant structure. The consequences of these chromosomal aberrations are difficult to study in cancer, and therefore several model systems have been developed in recent years. We show that human cells with extra chromosome engineered via microcell-mediated chromosome transfer often gain massive chromosomal rearrangements. The rearrangements arose by chromosome shattering and rejoining as well as by replication-dependent mechanisms. We show that the isolated micronuclei lack functional lamin B1 and become prone to envelope rupture, which leads to DNA damage and aberrant replication. The presence of functional lamin B1 partly correlates with micronuclei size, suggesting that the proper assembly of nuclear envelope might be sensitive to membrane curvature. The chromosomal rearrangements in trisomic cells provide growth advantage compared to cells without rearrangements. Our model system enables to study mechanisms of massive chromosomal rearrangements of any chromosome and their consequences in human cells.

Original languageEnglish
Article numbere50292
JournaleLife
Volume8
DOIs
Publication statusPublished - Nov 2019
Externally publishedYes

Keywords

  • Alzheimer’s disease
  • Cerebral organoids
  • HiPSC-derived neuronal cultures
  • Hyperexcitability

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