MiR-125b, miR-100 and miR-99a co-regulate vincristine resistance in childhood acute lymphoblastic leukemia

F Akbari Moqadam; E A M Lange-Turenhout; I M Ariës; R Pieters; M L den Boer

doi:10.1016/j.leukres.2013.06.027

MiR-125b, miR-100 and miR-99a co-regulate vincristine resistance in childhood acute lymphoblastic leukemia

F Akbari Moqadam
, E A M Lange-Turenhout
, I M Ariës
, R Pieters
, M L den Boer

Research output: Contribution to journal › Article › peer-review

74 Citations (Scopus)

Abstract

MicroRNA-125b (miR-125b), miR-99a and miR-100 are overexpressed in vincristine-resistant acute lymphoblastic leukemia (ALL). Cellular viability of ETV6-RUNX1-positive Reh cells significantly increased in presence of 9 ng/mL vincristine upon co-expression of miR-125b/miR-99a (91 ± 4%), miR-125b/miR-100 (93 ± 5%) or miR-125b/miR-99a/miR-100 (82 ± 17%) compared with miR-125b-transduced cells (38 ± 13%, P<0.05). Co-expression of these miRNAs resulted in downregulation of DNTT, NUCKS1, MALAT1, SNRPE, PNO1, SET, KIF5B, PRPS2, RPS11, RPL38 and RPL23A (fold-change 1.3-1.9, p<0.05). Similarly, 7 out of these genes are lower expressed in vincristine-resistant ALL cells of children (p<0.05). The concerted function of miR-125b in combination with miR-99a and/or miR-100 illustrates the complexity of vincristine-resistant pediatric ALL.

Original language	English
Pages (from-to)	1315-21
Number of pages	7
Journal	Leukemia research
Volume	37
Issue number	10
DOIs	https://doi.org/10.1016/j.leukres.2013.06.027
Publication status	Published - Oct 2013
Externally published	Yes

Keywords

Antineoplastic Agents, Phytogenic/pharmacology
Base Sequence
Child
Child, Preschool
Drug Resistance, Neoplasm/genetics
Gene Expression
Gene Expression Regulation, Leukemic
Gene Order
Humans
MicroRNAs/genetics
Precursor Cell Lymphoblastic Leukemia-Lymphoma/drug therapy
Vincristine/pharmacology

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10.1016/j.leukres.2013.06.027

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@article{07b0374f6d9b44929aa90bff9d822ee8,

title = "MiR-125b, miR-100 and miR-99a co-regulate vincristine resistance in childhood acute lymphoblastic leukemia",

abstract = "MicroRNA-125b (miR-125b), miR-99a and miR-100 are overexpressed in vincristine-resistant acute lymphoblastic leukemia (ALL). Cellular viability of ETV6-RUNX1-positive Reh cells significantly increased in presence of 9 ng/mL vincristine upon co-expression of miR-125b/miR-99a (91 ± 4\%), miR-125b/miR-100 (93 ± 5\%) or miR-125b/miR-99a/miR-100 (82 ± 17\%) compared with miR-125b-transduced cells (38 ± 13\%, P<0.05). Co-expression of these miRNAs resulted in downregulation of DNTT, NUCKS1, MALAT1, SNRPE, PNO1, SET, KIF5B, PRPS2, RPS11, RPL38 and RPL23A (fold-change 1.3-1.9, p<0.05). Similarly, 7 out of these genes are lower expressed in vincristine-resistant ALL cells of children (p<0.05). The concerted function of miR-125b in combination with miR-99a and/or miR-100 illustrates the complexity of vincristine-resistant pediatric ALL.",

keywords = "Antineoplastic Agents, Phytogenic/pharmacology, Base Sequence, Child, Child, Preschool, Drug Resistance, Neoplasm/genetics, Gene Expression, Gene Expression Regulation, Leukemic, Gene Order, Humans, MicroRNAs/genetics, Precursor Cell Lymphoblastic Leukemia-Lymphoma/drug therapy, Vincristine/pharmacology",

author = "\{Akbari Moqadam\}, F and Lange-Turenhout, \{E A M\} and Ari{\"e}s, \{I M\} and R Pieters and \{den Boer\}, \{M L\}",

year = "2013",

month = oct,

doi = "10.1016/j.leukres.2013.06.027",

language = "English",

volume = "37",

pages = "1315--21",

journal = "Leukemia research",

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publisher = "Elsevier Ltd.",

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TY - JOUR

T1 - MiR-125b, miR-100 and miR-99a co-regulate vincristine resistance in childhood acute lymphoblastic leukemia

AU - Akbari Moqadam, F

AU - Lange-Turenhout, E A M

AU - Ariës, I M

AU - Pieters, R

AU - den Boer, M L

PY - 2013/10

Y1 - 2013/10

N2 - MicroRNA-125b (miR-125b), miR-99a and miR-100 are overexpressed in vincristine-resistant acute lymphoblastic leukemia (ALL). Cellular viability of ETV6-RUNX1-positive Reh cells significantly increased in presence of 9 ng/mL vincristine upon co-expression of miR-125b/miR-99a (91 ± 4%), miR-125b/miR-100 (93 ± 5%) or miR-125b/miR-99a/miR-100 (82 ± 17%) compared with miR-125b-transduced cells (38 ± 13%, P<0.05). Co-expression of these miRNAs resulted in downregulation of DNTT, NUCKS1, MALAT1, SNRPE, PNO1, SET, KIF5B, PRPS2, RPS11, RPL38 and RPL23A (fold-change 1.3-1.9, p<0.05). Similarly, 7 out of these genes are lower expressed in vincristine-resistant ALL cells of children (p<0.05). The concerted function of miR-125b in combination with miR-99a and/or miR-100 illustrates the complexity of vincristine-resistant pediatric ALL.

AB - MicroRNA-125b (miR-125b), miR-99a and miR-100 are overexpressed in vincristine-resistant acute lymphoblastic leukemia (ALL). Cellular viability of ETV6-RUNX1-positive Reh cells significantly increased in presence of 9 ng/mL vincristine upon co-expression of miR-125b/miR-99a (91 ± 4%), miR-125b/miR-100 (93 ± 5%) or miR-125b/miR-99a/miR-100 (82 ± 17%) compared with miR-125b-transduced cells (38 ± 13%, P<0.05). Co-expression of these miRNAs resulted in downregulation of DNTT, NUCKS1, MALAT1, SNRPE, PNO1, SET, KIF5B, PRPS2, RPS11, RPL38 and RPL23A (fold-change 1.3-1.9, p<0.05). Similarly, 7 out of these genes are lower expressed in vincristine-resistant ALL cells of children (p<0.05). The concerted function of miR-125b in combination with miR-99a and/or miR-100 illustrates the complexity of vincristine-resistant pediatric ALL.

KW - Antineoplastic Agents, Phytogenic/pharmacology

KW - Base Sequence

KW - Child

KW - Child, Preschool

KW - Drug Resistance, Neoplasm/genetics

KW - Gene Expression

KW - Gene Expression Regulation, Leukemic

KW - Gene Order

KW - Humans

KW - MicroRNAs/genetics

KW - Precursor Cell Lymphoblastic Leukemia-Lymphoma/drug therapy

KW - Vincristine/pharmacology

UR - https://www.scopus.com/pages/publications/84883558416

U2 - 10.1016/j.leukres.2013.06.027

DO - 10.1016/j.leukres.2013.06.027

M3 - Article

C2 - 23915977

SN - 0145-2126

VL - 37

SP - 1315

EP - 1321

JO - Leukemia research

JF - Leukemia research

IS - 10

ER -

MiR-125b, miR-100 and miR-99a co-regulate vincristine resistance in childhood acute lymphoblastic leukemia

Abstract

Keywords

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