TY - JOUR
T1 - Modulation of cytarabine induced cytotoxicity using novel deoxynucleoside analogs in the HL60 cell line
AU - Hubeek, I.
AU - Peters, G. J.
AU - Broekhuizen, A. J.F.
AU - Kaspers, G. J.L.
PY - 2004
Y1 - 2004
N2 - In order to enhance the cytotoxicity of ara-C in the HL60 cell line the following deoxynucleoside analogs were used: cladribine, fludarabine and gemcitabine. HL60 cells were co-incubated with ara-C and each of the modulators at the ratios of their respective IC50s. Cytotoxicity was determined with the MTT-assay and drug interactions were evaluated with the combination index (CI) method (Calcusyn; Chou & Talalay). CI < 1, CI ± 1 and >1 indicate synergism, additive effect and antagonism, respectively. We observed moderate synergism between ara-C/cladribine and ara-C/ gemcitabine, with CIs of 0.76 ± 0.14 and 0.82 ± 0.04, respectively. The interaction between ara-C/fludarabine resulted in moderate antagonism (CI = 1.29 ± 0.11). In conclusion, in this in vitro study we showed that the cytotoxicity of ara-C can be succesfully modulated in the HL60 cell line by cladribine and gemcitabine.
AB - In order to enhance the cytotoxicity of ara-C in the HL60 cell line the following deoxynucleoside analogs were used: cladribine, fludarabine and gemcitabine. HL60 cells were co-incubated with ara-C and each of the modulators at the ratios of their respective IC50s. Cytotoxicity was determined with the MTT-assay and drug interactions were evaluated with the combination index (CI) method (Calcusyn; Chou & Talalay). CI < 1, CI ± 1 and >1 indicate synergism, additive effect and antagonism, respectively. We observed moderate synergism between ara-C/cladribine and ara-C/ gemcitabine, with CIs of 0.76 ± 0.14 and 0.82 ± 0.04, respectively. The interaction between ara-C/fludarabine resulted in moderate antagonism (CI = 1.29 ± 0.11). In conclusion, in this in vitro study we showed that the cytotoxicity of ara-C can be succesfully modulated in the HL60 cell line by cladribine and gemcitabine.
KW - Cytarabine
KW - Deoxynucleoside analogs
KW - Modulation
UR - http://www.scopus.com/inward/record.url?scp=10344222675&partnerID=8YFLogxK
U2 - 10.1081/NCN-200027727
DO - 10.1081/NCN-200027727
M3 - Article
C2 - 15571288
AN - SCOPUS:10344222675
SN - 1525-7770
VL - 23
SP - 1513
EP - 1516
JO - Nucleosides, Nucleotides and Nucleic Acids
JF - Nucleosides, Nucleotides and Nucleic Acids
IS - 8-9
ER -