Molecular characterization of a recurring complex chromosomal translocation in two human extragonadal germ cell tumors

R. J. Sinke; D. Olde Weghuis; R. F. Suijkerbuijk; A. Tanigami; Y. Nakamura; C. Larsson; G. Weber; B. de Jong; J. W. Oosterhuis; W. M. Molenaar; A. Geurts van Kessel

doi:10.1016/0165-4608(94)90176-7

Molecular characterization of a recurring complex chromosomal translocation in two human extragonadal germ cell tumors

R. J. Sinke, D. Olde Weghuis, R. F. Suijkerbuijk, A. Tanigami, Y. Nakamura, C. Larsson, G. Weber, B. de Jong, J. W. Oosterhuis, W. M. Molenaar, A. Geurts van Kessel

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Abstract

The molecular characterization of a recurring complex chromosomal translocation involving 6p21, 6p22, 6q23, and 11q13 in two independent but similar extragonadal human germ cell tumors was initiated using fluorescence in situ hybridization (FISH) and pulse field gel electrophoresis (PFGE) techniques. By using a series of specific probes from the 11q13 region, the translocation breakpoint in this chromosomal band could be located within a long-range restriction enzyme map in between the markers D11S457 and D11S546. In addition, aberrantly hybridizing restriction fragments were revealed by PFGE in both tumors, indicating that the breakpoint region must be located within a distance of at maximum 200 kilobase pairs (kbp) from the nearest DNA marker (D11S546).

Original language	English
Pages (from-to)	11-16
Number of pages	6
Journal	Cancer Genetics and Cytogenetics
Volume	73
Issue number	1
DOIs	https://doi.org/10.1016/0165-4608(94)90176-7
Publication status	Published - Mar 1994
Externally published	Yes

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Sinke, R. J., Olde Weghuis, D., Suijkerbuijk, R. F., Tanigami, A., Nakamura, Y., Larsson, C., Weber, G., de Jong, B., Oosterhuis, J. W., Molenaar, W. M., & Geurts van Kessel, A. (1994). Molecular characterization of a recurring complex chromosomal translocation in two human extragonadal germ cell tumors. Cancer Genetics and Cytogenetics, 73(1), 11-16. https://doi.org/10.1016/0165-4608(94)90176-7

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title = "Molecular characterization of a recurring complex chromosomal translocation in two human extragonadal germ cell tumors",

abstract = "The molecular characterization of a recurring complex chromosomal translocation involving 6p21, 6p22, 6q23, and 11q13 in two independent but similar extragonadal human germ cell tumors was initiated using fluorescence in situ hybridization (FISH) and pulse field gel electrophoresis (PFGE) techniques. By using a series of specific probes from the 11q13 region, the translocation breakpoint in this chromosomal band could be located within a long-range restriction enzyme map in between the markers D11S457 and D11S546. In addition, aberrantly hybridizing restriction fragments were revealed by PFGE in both tumors, indicating that the breakpoint region must be located within a distance of at maximum 200 kilobase pairs (kbp) from the nearest DNA marker (D11S546).",

author = "Sinke, {R. J.} and {Olde Weghuis}, D. and Suijkerbuijk, {R. F.} and A. Tanigami and Y. Nakamura and C. Larsson and G. Weber and {de Jong}, B. and Oosterhuis, {J. W.} and Molenaar, {W. M.} and {Geurts van Kessel}, A.",

year = "1994",

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Sinke, RJ, Olde Weghuis, D, Suijkerbuijk, RF, Tanigami, A, Nakamura, Y, Larsson, C, Weber, G, de Jong, B, Oosterhuis, JW, Molenaar, WM & Geurts van Kessel, A 1994, 'Molecular characterization of a recurring complex chromosomal translocation in two human extragonadal germ cell tumors', Cancer Genetics and Cytogenetics, vol. 73, no. 1, pp. 11-16. https://doi.org/10.1016/0165-4608(94)90176-7

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T1 - Molecular characterization of a recurring complex chromosomal translocation in two human extragonadal germ cell tumors

AU - Sinke, R. J.

AU - Olde Weghuis, D.

AU - Suijkerbuijk, R. F.

AU - Tanigami, A.

AU - Nakamura, Y.

AU - Larsson, C.

AU - Weber, G.

AU - de Jong, B.

AU - Oosterhuis, J. W.

AU - Molenaar, W. M.

AU - Geurts van Kessel, A.

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N2 - The molecular characterization of a recurring complex chromosomal translocation involving 6p21, 6p22, 6q23, and 11q13 in two independent but similar extragonadal human germ cell tumors was initiated using fluorescence in situ hybridization (FISH) and pulse field gel electrophoresis (PFGE) techniques. By using a series of specific probes from the 11q13 region, the translocation breakpoint in this chromosomal band could be located within a long-range restriction enzyme map in between the markers D11S457 and D11S546. In addition, aberrantly hybridizing restriction fragments were revealed by PFGE in both tumors, indicating that the breakpoint region must be located within a distance of at maximum 200 kilobase pairs (kbp) from the nearest DNA marker (D11S546).

AB - The molecular characterization of a recurring complex chromosomal translocation involving 6p21, 6p22, 6q23, and 11q13 in two independent but similar extragonadal human germ cell tumors was initiated using fluorescence in situ hybridization (FISH) and pulse field gel electrophoresis (PFGE) techniques. By using a series of specific probes from the 11q13 region, the translocation breakpoint in this chromosomal band could be located within a long-range restriction enzyme map in between the markers D11S457 and D11S546. In addition, aberrantly hybridizing restriction fragments were revealed by PFGE in both tumors, indicating that the breakpoint region must be located within a distance of at maximum 200 kilobase pairs (kbp) from the nearest DNA marker (D11S546).

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JF - Cancer Genetics and Cytogenetics

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Molecular characterization of a recurring complex chromosomal translocation in two human extragonadal germ cell tumors

Abstract

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