Molecular cloning of a human DNA repair gene

A. Westerveld; J. H.J. Hoeijmakers; M. Van Duin; J. De Wit; H. Odijk; A. Pastink; R. D. Wood; D. Bootsma

doi:10.1038/310425a0

Molecular cloning of a human DNA repair gene

A. Westerveld, J. H.J. Hoeijmakers, M. Van Duin, J. De Wit, H. Odijk, A. Pastink, R. D. Wood, D. Bootsma

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8 Citations (Scopus)

Abstract

Cell strains derived from patients having a hereditary disorder associated with defects in repair of DNA damage such as xeroderma pigmentosum^1,2 and mutants isolated from established rodent cell lines^3,4 provide the tools for genetic and biochemical analysis of DNA repair pathways in mammalian cells. Complementation studies using these cells have illustrated the genetic and biochemical complexity of these pathways^3,5,6. The precise nature of the genes and gene products involved in these mutants has not yet been resolved. Isolation of repair genes by recombinant DNA technology would open up new approaches to the elucidation of repair mechanisms in mammalian cells. Here we report the molecular cloning of a human repair gene (ERCC1) that complements the repair defect in a Chinese hamster ovary (CHO) mutant cell line.

Original language	English
Pages (from-to)	425-429
Number of pages	5
Journal	Nature
Volume	310
Issue number	5976
DOIs	https://doi.org/10.1038/310425a0
Publication status	Published - 1984
Externally published	Yes

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title = "Molecular cloning of a human DNA repair gene",

abstract = "Cell strains derived from patients having a hereditary disorder associated with defects in repair of DNA damage such as xeroderma pigmentosum1,2 and mutants isolated from established rodent cell lines3,4 provide the tools for genetic and biochemical analysis of DNA repair pathways in mammalian cells. Complementation studies using these cells have illustrated the genetic and biochemical complexity of these pathways3,5,6. The precise nature of the genes and gene products involved in these mutants has not yet been resolved. Isolation of repair genes by recombinant DNA technology would open up new approaches to the elucidation of repair mechanisms in mammalian cells. Here we report the molecular cloning of a human repair gene (ERCC1) that complements the repair defect in a Chinese hamster ovary (CHO) mutant cell line.",

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year = "1984",

doi = "10.1038/310425a0",

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journal = "Nature",

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T1 - Molecular cloning of a human DNA repair gene

AU - Westerveld, A.

AU - Hoeijmakers, J. H.J.

AU - Van Duin, M.

AU - De Wit, J.

AU - Odijk, H.

AU - Pastink, A.

AU - Wood, R. D.

AU - Bootsma, D.

PY - 1984

Y1 - 1984

N2 - Cell strains derived from patients having a hereditary disorder associated with defects in repair of DNA damage such as xeroderma pigmentosum1,2 and mutants isolated from established rodent cell lines3,4 provide the tools for genetic and biochemical analysis of DNA repair pathways in mammalian cells. Complementation studies using these cells have illustrated the genetic and biochemical complexity of these pathways3,5,6. The precise nature of the genes and gene products involved in these mutants has not yet been resolved. Isolation of repair genes by recombinant DNA technology would open up new approaches to the elucidation of repair mechanisms in mammalian cells. Here we report the molecular cloning of a human repair gene (ERCC1) that complements the repair defect in a Chinese hamster ovary (CHO) mutant cell line.

AB - Cell strains derived from patients having a hereditary disorder associated with defects in repair of DNA damage such as xeroderma pigmentosum1,2 and mutants isolated from established rodent cell lines3,4 provide the tools for genetic and biochemical analysis of DNA repair pathways in mammalian cells. Complementation studies using these cells have illustrated the genetic and biochemical complexity of these pathways3,5,6. The precise nature of the genes and gene products involved in these mutants has not yet been resolved. Isolation of repair genes by recombinant DNA technology would open up new approaches to the elucidation of repair mechanisms in mammalian cells. Here we report the molecular cloning of a human repair gene (ERCC1) that complements the repair defect in a Chinese hamster ovary (CHO) mutant cell line.

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SN - 0028-0836

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Molecular cloning of a human DNA repair gene

Abstract

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