Naturally acquired microchimerism implications for transplantation outcome and novel methodologies for detection

Michael Eikmans, Astrid G.S. van Halteren, Koen van Besien, Jon J. van Rood, Jos J.M. Drabbels, Frans H.J. Claas

Research output: Contribution to journalReview articlepeer-review

39 Citations (Scopus)

Abstract

Microchimerism represents a condition where one individual harbors genetically distinct cell populations, and the chimeric population constitutes <1% of the total number of cells. The most common natural source of microchimerism is pregnancy. The reciprocal cell exchange between a mother and her child often leads to the stable engraftment of hematopoietic and non-hematopoietic stem cells in both parties. Interaction between cells from the mother and those from the child may result in maternal immune cells becoming sensitized to inherited paternal alloantigens of the child, which are not expressed by the mother herself. Vice versa, immune cells of the child may become sensitized toward the non-inherited maternal alloantigens of the mother. The extent of microchimerism, its anatomical location, and the sensitivity of the techniques used for detecting its presence collectively determine whether microchimerism can be detected in an individual. In this review, we focus on the clinical consequences of microchimerism in solid organ and hematopoietic stem cell transplantation, and propose concepts derived from data of epidemiologic studies. Next, we elaborate on the latest molecular methodology, including digital PCR, for determining in a reliable and sensitive way the extent of microchimerism. For the first time, tools have become available to isolate viable chimeric cells from a host background, so that the challenges of establishing the biologic mechanisms and function of these cells may finally be tackled.

Original languageEnglish
JournalChimerism
Volume5
Issue number2
DOIs
Publication statusPublished - 2014
Externally publishedYes

Keywords

  • Digital PCR
  • FACS sorting
  • Graft-versus-host disease
  • Maternal antigen
  • Microchimerism
  • Mixed chimerism
  • Monoclonal antibodies
  • Paternal antigen
  • Pregnancy
  • qPCR
  • Single cell analysis
  • Transplantation

Fingerprint

Dive into the research topics of 'Naturally acquired microchimerism implications for transplantation outcome and novel methodologies for detection'. Together they form a unique fingerprint.

Cite this