TY - JOUR
T1 - Nosocomial transmission of NDM-1-containing Klebsiella pneumoniae ST147 in a Dutch pediatric oncology center associated with patients from Ukraine
AU - Dutch CPE Surveillance Study Group
AU - Smit, Wouter L.
AU - Wunderink, Herman F.
AU - Kluytmans, Jan A.J.W.
AU - Tissing, Wim J.E.
AU - van Dijkhuizen, E. H.Pieter
AU - Loeffen, Yvette G.T.
AU - Hendrickx, Antoni P.A.
AU - Steingrover, R.
AU - de Brauwer, E. I.G.B.
AU - van Dam, D. W.
AU - de Vries, J.
AU - Bathoorn, E.
AU - Troelstra, A.
AU - Dorigo-Zetsma, J. W.
AU - Reijer, M. den
AU - Heikens, E.
AU - Vainio, S. J.
AU - de Jong, E.
AU - de Graaf, M.
AU - van Meer, M. P.A.
AU - van der Vorm, E.
AU - Sinnige, J. C.
AU - Maraha, B.
AU - Maat, I.
AU - van Dam, A.
AU - Gruteke, P.
AU - Stuart, J. W.T.Cohen
AU - Melles, D. C.
AU - van Mens, S. P.
AU - Dinant, S.
AU - Schulin, T.
AU - Al Naiemi, N.
AU - Kolwijck, E.
AU - Debast, S. B.
AU - van Rijn, M.
AU - van der Linden, M. P.M.
AU - Muller, A. E.
AU - Wong, M.
AU - van Trijp, M. J.C.A.
AU - Foe, J. R.Lo Ten
AU - van Griethuysen, A. J.
AU - Paltansing, S.
AU - Jansz, A.
AU - Bode, L. G.M.
AU - Buiting, A. G.M.
AU - Vlek, A. L.M.
AU - da Silva, J.
AU - Ang, W.
AU - Waar, K.
AU - Ott, A.
N1 - Publisher Copyright:
© The Author(s) 2024.
PY - 2024/12
Y1 - 2024/12
N2 - We studied nosocomial transmission of multidrug-resistant blaNDM-1-containing Klebsiella pneumoniae ST147 in a Dutch pediatric oncology center. Whole-genome multilocus sequence typing revealed two genetic clusters consisting of 2 and 5 K. pneumoniae isolates, both from Ukrainian medical evacuees and Dutch patients. Ukrainian patients were colonized at admission, and two Dutch patients developed bacteremia. Temporal and spatial transmission links were assessed and sampling of sinks revealed environmental contamination of blaNDM-1-containing K. pneumoniae with genetic relatedness to cluster isolates. Phylogenetic analysis of 163 K. pneumoniae ST147 isolates from the Dutch national surveillance revealed that transmission-related isolates formed a unique phylogenetic branch. In conclusion, we demonstrate nosocomial transmission of unique blaNDM-1-containing K. pneumoniae ST147 strains introduced by Ukrainian patients. Sink drains colonized by genetically similar blaNDM-1-containing strains in rooms designated as spatial links within the cluster were considered the potential source of transmission. Healthcare professionals should be aware of these risks, particularly in settings with susceptible patients exposed to antibiotic pressure.
AB - We studied nosocomial transmission of multidrug-resistant blaNDM-1-containing Klebsiella pneumoniae ST147 in a Dutch pediatric oncology center. Whole-genome multilocus sequence typing revealed two genetic clusters consisting of 2 and 5 K. pneumoniae isolates, both from Ukrainian medical evacuees and Dutch patients. Ukrainian patients were colonized at admission, and two Dutch patients developed bacteremia. Temporal and spatial transmission links were assessed and sampling of sinks revealed environmental contamination of blaNDM-1-containing K. pneumoniae with genetic relatedness to cluster isolates. Phylogenetic analysis of 163 K. pneumoniae ST147 isolates from the Dutch national surveillance revealed that transmission-related isolates formed a unique phylogenetic branch. In conclusion, we demonstrate nosocomial transmission of unique blaNDM-1-containing K. pneumoniae ST147 strains introduced by Ukrainian patients. Sink drains colonized by genetically similar blaNDM-1-containing strains in rooms designated as spatial links within the cluster were considered the potential source of transmission. Healthcare professionals should be aware of these risks, particularly in settings with susceptible patients exposed to antibiotic pressure.
KW - Environmental contamination
KW - Medical evacuees
KW - NDM-1-containing Klebsiella pneumoniae
KW - Nosocomial transmission
UR - https://www.scopus.com/pages/publications/85212941478
U2 - 10.1186/s12879-024-10368-2
DO - 10.1186/s12879-024-10368-2
M3 - Article
C2 - 39716112
AN - SCOPUS:85212941478
SN - 1471-2334
VL - 24
JO - BMC Infectious Diseases
JF - BMC Infectious Diseases
IS - 1
M1 - 1460
ER -