Novel monoclonal antibodies against membrane structures that are preferentially expressed on IL-2-activated rat NK cells

Katinka M. Giezeman-Smits, Liesbeth E. Jonges, William H. Chambers, Cynthia S. Brisette-Storkus, Ronald L.P. Van Vlierberghe, Jaap D.H. Van Eendenburg, Alexander M.M. Eggermont, Gert Jan Fleuren, Peter J.K. Kuppen

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

Interleukin-2 (IL-2)-activated natural killer (NK) cells are known to mediate specific functions such as cytolytic activity and tumor infiltration more efficiently than nonactivated NK cells. To investigate whether these characteristics are associated with induction or up-regulation of expression of membrane structures after IL-2 activation, we selected four hybridomas (mAbs ANK44, ANK66, ANK7, and ANK123) derived from mice immunized with rat IL-2-activated NK cells and compared the expression of the epitopes recognized on IL-2-activated NK cells versus unstimulated NK cells. We found that ANK44-expression was induced after activation with IL-2. The antigens recognized by ANK66, ANK7, and ANK123 were expressed selectively on subsets of nonactivated NK cells. After activation with IL-2 the level of expression of these antigens was increased. Moreover, the majority of NK cells then expressed these antigens after IL-2 activation. Biochemical characterization of the membrane structures recognized on IL-2-activated NK cells showed that they have not previously been described. The precise function of these membrane structures is not yet known, however, the selective nature of their expression implies their involvement in the specific functions.

Original languageEnglish
Pages (from-to)209-215
Number of pages7
JournalJournal of Leukocyte Biology
Volume63
Issue number2
DOIs
Publication statusPublished - Feb 1998
Externally publishedYes

Keywords

  • Cytolytic activity
  • Hybridomas
  • Tumor infiltration

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