Online fluorescent method to assess BCRP/ABCG2 activity in suspension cells

J. H. Hooijberg; G. J. Peters; G. J.L. Kaspers; P. R. Wielinga; A. J.P. Veerman; R. Pieters; G. Jansen

doi:10.1081/NCN-200027672

Online fluorescent method to assess BCRP/ABCG2 activity in suspension cells

J. H. Hooijberg
, G. J. Peters
, G. J.L. Kaspers
, P. R. Wielinga
, A. J.P. Veerman
, R. Pieters
, G. Jansen

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

An online method was developed to monitor BCRP mediated efflux of fluorescent substrates in suspension cells. To this end, a 2-compartment system consisting of a transwell cup and a cuvette was used. In this system we were able to observe differences in efflux kinetics between BCRP overexpressing RPMI 8226/MR cells and parental myeloid RPMI 8226(s) cells using only 50,000 cells per experiment. 8226/MR cells displayed a larger cellular efflux rate of the BCRP substrate Hoechst 33342, as compared to the wildtype cells. This difference in efflux rate was completely decreased in the presence of the BCRP inhibitor Ko143.

Original language	English
Pages (from-to)	1451-1454
Number of pages	4
Journal	Nucleosides, Nucleotides and Nucleic Acids
Volume	23
Issue number	8-9
DOIs	https://doi.org/10.1081/NCN-200027672
Publication status	Published - 2004
Externally published	Yes

Keywords

ABC-transporters
BCRP/ABCG2
Efflux rate
Hoechst 33342
Kol43

Access to Document

10.1081/NCN-200027672

Cite this

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title = "Online fluorescent method to assess BCRP/ABCG2 activity in suspension cells",

abstract = "An online method was developed to monitor BCRP mediated efflux of fluorescent substrates in suspension cells. To this end, a 2-compartment system consisting of a transwell cup and a cuvette was used. In this system we were able to observe differences in efflux kinetics between BCRP overexpressing RPMI 8226/MR cells and parental myeloid RPMI 8226(s) cells using only 50,000 cells per experiment. 8226/MR cells displayed a larger cellular efflux rate of the BCRP substrate Hoechst 33342, as compared to the wildtype cells. This difference in efflux rate was completely decreased in the presence of the BCRP inhibitor Ko143.",

keywords = "ABC-transporters, BCRP/ABCG2, Efflux rate, Hoechst 33342, Kol43",

author = "Hooijberg, \{J. H.\} and Peters, \{G. J.\} and Kaspers, \{G. J.L.\} and Wielinga, \{P. R.\} and Veerman, \{A. J.P.\} and R. Pieters and G. Jansen",

note = "Funding Information: Supported by the Dutch Cancer Society, grant VU-2000-2237.",

year = "2004",

doi = "10.1081/NCN-200027672",

language = "English",

volume = "23",

pages = "1451--1454",

journal = "Nucleosides, Nucleotides and Nucleic Acids",

issn = "1525-7770",

publisher = "Taylor and Francis Ltd.",

number = "8-9",

}

TY - JOUR

T1 - Online fluorescent method to assess BCRP/ABCG2 activity in suspension cells

AU - Hooijberg, J. H.

AU - Peters, G. J.

AU - Kaspers, G. J.L.

AU - Wielinga, P. R.

AU - Veerman, A. J.P.

AU - Pieters, R.

AU - Jansen, G.

N1 - Funding Information: Supported by the Dutch Cancer Society, grant VU-2000-2237.

PY - 2004

Y1 - 2004

N2 - An online method was developed to monitor BCRP mediated efflux of fluorescent substrates in suspension cells. To this end, a 2-compartment system consisting of a transwell cup and a cuvette was used. In this system we were able to observe differences in efflux kinetics between BCRP overexpressing RPMI 8226/MR cells and parental myeloid RPMI 8226(s) cells using only 50,000 cells per experiment. 8226/MR cells displayed a larger cellular efflux rate of the BCRP substrate Hoechst 33342, as compared to the wildtype cells. This difference in efflux rate was completely decreased in the presence of the BCRP inhibitor Ko143.

AB - An online method was developed to monitor BCRP mediated efflux of fluorescent substrates in suspension cells. To this end, a 2-compartment system consisting of a transwell cup and a cuvette was used. In this system we were able to observe differences in efflux kinetics between BCRP overexpressing RPMI 8226/MR cells and parental myeloid RPMI 8226(s) cells using only 50,000 cells per experiment. 8226/MR cells displayed a larger cellular efflux rate of the BCRP substrate Hoechst 33342, as compared to the wildtype cells. This difference in efflux rate was completely decreased in the presence of the BCRP inhibitor Ko143.

KW - ABC-transporters

KW - BCRP/ABCG2

KW - Efflux rate

KW - Hoechst 33342

KW - Kol43

UR - https://www.scopus.com/pages/publications/10344222677

U2 - 10.1081/NCN-200027672

DO - 10.1081/NCN-200027672

M3 - Article

C2 - 15571275

AN - SCOPUS:10344222677

SN - 1525-7770

VL - 23

SP - 1451

EP - 1454

JO - Nucleosides, Nucleotides and Nucleic Acids

JF - Nucleosides, Nucleotides and Nucleic Acids

IS - 8-9

ER -

Online fluorescent method to assess BCRP/ABCG2 activity in suspension cells

Abstract

Keywords

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