Oxidative Stress, DNA, Cell Cycle/Cell Cycle Associated Proteins and Multidrug Resistance Proteins: Targets of Human Amniotic Membrane in Hepatocellular Carcinoma

A C Mamede, S Guerra, M Laranjo, K Santos, M J Carvalho, T Carvalheiro, P Moura, A Paiva, A M Abrantes, C J Maia, M F Botelho

Research output: Contribution to journalArticlepeer-review

Abstract

The anticancer effects of human amniotic membrane (hAM) have been studied over the last decade. However, the action mechanisms responsible for these effects are not fully understood until now. Previously results reported by our team proved that hAM is able to induce cytotoxicity and cell death in hepatocellular carcinoma (HCC), a worldwide high incident and mortal cancer. Therefore, this experimental study aimed to investigate the cellular targets of hAM protein extracts (hAMPE) in HCC through in vitro studies. Our results showed that hAMPE is able to modify oxidative stress environment in all HCC cell lines, as well as its cell cycle. hAMPE differently targets deoxyribonucleic acid (DNA), P21, P53, β-catenin and multidrug resistance (MDR) proteins in HCC cell lines. In conclusion, hAMPE has several targets in HCC, being clear that the success of this treatment depends of a personalized therapy based on the biological and genetic characteristics of the tumor.

Original languageEnglish
Pages (from-to)689-97
Number of pages9
JournalPathology oncology research : POR
Volume22
Issue number4
DOIs
Publication statusPublished - Oct 2016
Externally publishedYes

Keywords

  • ATP Binding Cassette Transporter, Subfamily B/metabolism
  • Amnion/metabolism
  • Antineoplastic Agents/pharmacology
  • Apoptosis/drug effects
  • Carcinoma, Hepatocellular/drug therapy
  • Cell Cycle/drug effects
  • Cell Cycle Proteins/metabolism
  • Cell Line, Tumor
  • Cyclin-Dependent Kinase Inhibitor p21/metabolism
  • DNA/metabolism
  • Hep G2 Cells
  • Humans
  • Liver Neoplasms/drug therapy
  • Membrane Proteins/metabolism
  • Oxidative Stress/drug effects
  • Tumor Suppressor Protein p53/metabolism
  • beta Catenin/metabolism

Fingerprint

Dive into the research topics of 'Oxidative Stress, DNA, Cell Cycle/Cell Cycle Associated Proteins and Multidrug Resistance Proteins: Targets of Human Amniotic Membrane in Hepatocellular Carcinoma'. Together they form a unique fingerprint.

Cite this