Oxidative Stress, DNA, Cell Cycle/Cell Cycle Associated Proteins and Multidrug Resistance Proteins: Targets of Human Amniotic Membrane in Hepatocellular Carcinoma

A C Mamede; S Guerra; M Laranjo; K Santos; M J Carvalho; T Carvalheiro; P Moura; A Paiva; A M Abrantes; C J Maia; M F Botelho

doi:10.1007/s12253-016-0053-x

Oxidative Stress, DNA, Cell Cycle/Cell Cycle Associated Proteins and Multidrug Resistance Proteins: Targets of Human Amniotic Membrane in Hepatocellular Carcinoma

A C Mamede, S Guerra, M Laranjo, K Santos, M J Carvalho, T Carvalheiro, P Moura, A Paiva, A M Abrantes, C J Maia, M F Botelho

Research output: Contribution to journal › Article › peer-review

17 Citations (Scopus)

Abstract

The anticancer effects of human amniotic membrane (hAM) have been studied over the last decade. However, the action mechanisms responsible for these effects are not fully understood until now. Previously results reported by our team proved that hAM is able to induce cytotoxicity and cell death in hepatocellular carcinoma (HCC), a worldwide high incident and mortal cancer. Therefore, this experimental study aimed to investigate the cellular targets of hAM protein extracts (hAMPE) in HCC through in vitro studies. Our results showed that hAMPE is able to modify oxidative stress environment in all HCC cell lines, as well as its cell cycle. hAMPE differently targets deoxyribonucleic acid (DNA), P21, P53, β-catenin and multidrug resistance (MDR) proteins in HCC cell lines. In conclusion, hAMPE has several targets in HCC, being clear that the success of this treatment depends of a personalized therapy based on the biological and genetic characteristics of the tumor.

Original language	English
Pages (from-to)	689-97
Number of pages	9
Journal	Pathology oncology research : POR
Volume	22
Issue number	4
DOIs	https://doi.org/10.1007/s12253-016-0053-x
Publication status	Published - Oct 2016
Externally published	Yes

Keywords

ATP Binding Cassette Transporter, Subfamily B/metabolism
Amnion/metabolism
Antineoplastic Agents/pharmacology
Apoptosis/drug effects
Carcinoma, Hepatocellular/drug therapy
Cell Cycle/drug effects
Cell Cycle Proteins/metabolism
Cell Line, Tumor
Cyclin-Dependent Kinase Inhibitor p21/metabolism
DNA/metabolism
Hep G2 Cells
Humans
Liver Neoplasms/drug therapy
Membrane Proteins/metabolism
Oxidative Stress/drug effects
Tumor Suppressor Protein p53/metabolism
beta Catenin/metabolism

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Mamede, A. C., Guerra, S., Laranjo, M., Santos, K., Carvalho, M. J., Carvalheiro, T., Moura, P., Paiva, A., Abrantes, A. M., Maia, C. J., & Botelho, M. F. (2016). Oxidative Stress, DNA, Cell Cycle/Cell Cycle Associated Proteins and Multidrug Resistance Proteins: Targets of Human Amniotic Membrane in Hepatocellular Carcinoma. Pathology oncology research : POR, 22(4), 689-97. https://doi.org/10.1007/s12253-016-0053-x

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title = "Oxidative Stress, DNA, Cell Cycle/Cell Cycle Associated Proteins and Multidrug Resistance Proteins: Targets of Human Amniotic Membrane in Hepatocellular Carcinoma",

abstract = "The anticancer effects of human amniotic membrane (hAM) have been studied over the last decade. However, the action mechanisms responsible for these effects are not fully understood until now. Previously results reported by our team proved that hAM is able to induce cytotoxicity and cell death in hepatocellular carcinoma (HCC), a worldwide high incident and mortal cancer. Therefore, this experimental study aimed to investigate the cellular targets of hAM protein extracts (hAMPE) in HCC through in vitro studies. Our results showed that hAMPE is able to modify oxidative stress environment in all HCC cell lines, as well as its cell cycle. hAMPE differently targets deoxyribonucleic acid (DNA), P21, P53, β-catenin and multidrug resistance (MDR) proteins in HCC cell lines. In conclusion, hAMPE has several targets in HCC, being clear that the success of this treatment depends of a personalized therapy based on the biological and genetic characteristics of the tumor. ",

keywords = "ATP Binding Cassette Transporter, Subfamily B/metabolism, Amnion/metabolism, Antineoplastic Agents/pharmacology, Apoptosis/drug effects, Carcinoma, Hepatocellular/drug therapy, Cell Cycle/drug effects, Cell Cycle Proteins/metabolism, Cell Line, Tumor, Cyclin-Dependent Kinase Inhibitor p21/metabolism, DNA/metabolism, Hep G2 Cells, Humans, Liver Neoplasms/drug therapy, Membrane Proteins/metabolism, Oxidative Stress/drug effects, Tumor Suppressor Protein p53/metabolism, beta Catenin/metabolism",

author = "Mamede, {A C} and S Guerra and M Laranjo and K Santos and Carvalho, {M J} and T Carvalheiro and P Moura and A Paiva and Abrantes, {A M} and Maia, {C J} and Botelho, {M F}",

note = "Publisher Copyright: {\textcopyright} 2016, Ar{\'a}nyi Lajos Foundation.",

year = "2016",

month = oct,

doi = "10.1007/s12253-016-0053-x",

language = "English",

volume = "22",

pages = "689--97",

journal = "Pathology oncology research : POR",

issn = "1219-4956",

publisher = "Frontiers Media SA",

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Mamede, AC, Guerra, S, Laranjo, M, Santos, K, Carvalho, MJ, Carvalheiro, T, Moura, P, Paiva, A, Abrantes, AM, Maia, CJ & Botelho, MF 2016, 'Oxidative Stress, DNA, Cell Cycle/Cell Cycle Associated Proteins and Multidrug Resistance Proteins: Targets of Human Amniotic Membrane in Hepatocellular Carcinoma', Pathology oncology research : POR, vol. 22, no. 4, pp. 689-97. https://doi.org/10.1007/s12253-016-0053-x

TY - JOUR

T1 - Oxidative Stress, DNA, Cell Cycle/Cell Cycle Associated Proteins and Multidrug Resistance Proteins

T2 - Targets of Human Amniotic Membrane in Hepatocellular Carcinoma

AU - Mamede, A C

AU - Guerra, S

AU - Laranjo, M

AU - Santos, K

AU - Carvalho, M J

AU - Carvalheiro, T

AU - Moura, P

AU - Paiva, A

AU - Abrantes, A M

AU - Maia, C J

AU - Botelho, M F

PY - 2016/10

Y1 - 2016/10

N2 - The anticancer effects of human amniotic membrane (hAM) have been studied over the last decade. However, the action mechanisms responsible for these effects are not fully understood until now. Previously results reported by our team proved that hAM is able to induce cytotoxicity and cell death in hepatocellular carcinoma (HCC), a worldwide high incident and mortal cancer. Therefore, this experimental study aimed to investigate the cellular targets of hAM protein extracts (hAMPE) in HCC through in vitro studies. Our results showed that hAMPE is able to modify oxidative stress environment in all HCC cell lines, as well as its cell cycle. hAMPE differently targets deoxyribonucleic acid (DNA), P21, P53, β-catenin and multidrug resistance (MDR) proteins in HCC cell lines. In conclusion, hAMPE has several targets in HCC, being clear that the success of this treatment depends of a personalized therapy based on the biological and genetic characteristics of the tumor.

AB - The anticancer effects of human amniotic membrane (hAM) have been studied over the last decade. However, the action mechanisms responsible for these effects are not fully understood until now. Previously results reported by our team proved that hAM is able to induce cytotoxicity and cell death in hepatocellular carcinoma (HCC), a worldwide high incident and mortal cancer. Therefore, this experimental study aimed to investigate the cellular targets of hAM protein extracts (hAMPE) in HCC through in vitro studies. Our results showed that hAMPE is able to modify oxidative stress environment in all HCC cell lines, as well as its cell cycle. hAMPE differently targets deoxyribonucleic acid (DNA), P21, P53, β-catenin and multidrug resistance (MDR) proteins in HCC cell lines. In conclusion, hAMPE has several targets in HCC, being clear that the success of this treatment depends of a personalized therapy based on the biological and genetic characteristics of the tumor.

KW - ATP Binding Cassette Transporter, Subfamily B/metabolism

KW - Amnion/metabolism

KW - Antineoplastic Agents/pharmacology

KW - Apoptosis/drug effects

KW - Carcinoma, Hepatocellular/drug therapy

KW - Cell Cycle/drug effects

KW - Cell Cycle Proteins/metabolism

KW - Cell Line, Tumor

KW - Cyclin-Dependent Kinase Inhibitor p21/metabolism

KW - DNA/metabolism

KW - Hep G2 Cells

KW - Humans

KW - Liver Neoplasms/drug therapy

KW - Membrane Proteins/metabolism

KW - Oxidative Stress/drug effects

KW - Tumor Suppressor Protein p53/metabolism

KW - beta Catenin/metabolism

UR - http://www.scopus.com/inward/record.url?scp=84960423275&partnerID=8YFLogxK

U2 - 10.1007/s12253-016-0053-x

DO - 10.1007/s12253-016-0053-x

M3 - Article

C2 - 26965246

SN - 1219-4956

VL - 22

SP - 689

EP - 697

JO - Pathology oncology research : POR

JF - Pathology oncology research : POR

IS - 4

ER -

Oxidative Stress, DNA, Cell Cycle/Cell Cycle Associated Proteins and Multidrug Resistance Proteins: Targets of Human Amniotic Membrane in Hepatocellular Carcinoma

Abstract

Keywords

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