Abstract
The frequency and function of T cells, monocytes, and dendritic cell subsets were investigated in 12 patients after acute myocardial infarction (AMI)-(T0), 1 month after the episode (T1), and in 12 healthy individuals (HG). The cell characterization and the functional studies were performed by flow cytometry and by RT-PCR, after cell sorting. The most important findings at T0 moment, when compared with T1 and HG, were: a decrease in the frequency of IL-2-producing T cells; a lower frequency of TNF-α- and IL-6-producing monocytes, myeloid dendritic cells, and CD14(-/low)CD16(+)DCs; and a lower TNF-α mRNA expression, after sorting these cells. Moreover, the regulatory function of Treg cells, at T0 moment, was upregulated, based on the FoxP3, CTLA-4, and TGF-β mRNA expression increase. The majority of these phenotypic and functional alterations disappeared at T1. Our data demonstrate that AMI induces a significant change in the immune system homeostasis.
| Original language | English |
|---|---|
| Pages (from-to) | 309-20 |
| Number of pages | 12 |
| Journal | Journal of cardiovascular translational research |
| Volume | 5 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Jun 2012 |
| Externally published | Yes |
Keywords
- Adult
- Aged
- Biomarkers/blood
- Case-Control Studies
- Cell Separation
- Dendritic Cells/immunology
- Female
- Flow Cytometry
- GPI-Linked Proteins/blood
- Humans
- Immunophenotyping
- Inflammation/blood
- Inflammation Mediators/blood
- Interleukin-2/blood
- Interleukin-6/blood
- Lipopolysaccharide Receptors/blood
- Male
- Middle Aged
- Monocytes/immunology
- Myocardial Infarction/blood
- Phenotype
- Portugal
- Prospective Studies
- RNA, Messenger/blood
- Real-Time Polymerase Chain Reaction
- Receptors, IgG/blood
- T-Lymphocyte Subsets/immunology
- Time Factors
- Tumor Necrosis Factor-alpha/blood