Phenotypic and functional alterations on inflammatory peripheral blood cells after acute myocardial infarction

Tiago Carvalheiro, Isabel Velada, Ana Valado, Fernando Mendes, António Martinho, Natália António, Lino Gonçalves, Luís Providência, Maria Luísa Pais, Artur Paiva

Research output: Contribution to journalArticlepeer-review

Abstract

The frequency and function of T cells, monocytes, and dendritic cell subsets were investigated in 12 patients after acute myocardial infarction (AMI)-(T0), 1 month after the episode (T1), and in 12 healthy individuals (HG). The cell characterization and the functional studies were performed by flow cytometry and by RT-PCR, after cell sorting. The most important findings at T0 moment, when compared with T1 and HG, were: a decrease in the frequency of IL-2-producing T cells; a lower frequency of TNF-α- and IL-6-producing monocytes, myeloid dendritic cells, and CD14(-/low)CD16(+)DCs; and a lower TNF-α mRNA expression, after sorting these cells. Moreover, the regulatory function of Treg cells, at T0 moment, was upregulated, based on the FoxP3, CTLA-4, and TGF-β mRNA expression increase. The majority of these phenotypic and functional alterations disappeared at T1. Our data demonstrate that AMI induces a significant change in the immune system homeostasis.

Original languageEnglish
Pages (from-to)309-20
Number of pages12
JournalJournal of cardiovascular translational research
Volume5
Issue number3
DOIs
Publication statusPublished - Jun 2012
Externally publishedYes

Keywords

  • Adult
  • Aged
  • Biomarkers/blood
  • Case-Control Studies
  • Cell Separation
  • Dendritic Cells/immunology
  • Female
  • Flow Cytometry
  • GPI-Linked Proteins/blood
  • Humans
  • Immunophenotyping
  • Inflammation/blood
  • Inflammation Mediators/blood
  • Interleukin-2/blood
  • Interleukin-6/blood
  • Lipopolysaccharide Receptors/blood
  • Male
  • Middle Aged
  • Monocytes/immunology
  • Myocardial Infarction/blood
  • Phenotype
  • Portugal
  • Prospective Studies
  • RNA, Messenger/blood
  • Real-Time Polymerase Chain Reaction
  • Receptors, IgG/blood
  • T-Lymphocyte Subsets/immunology
  • Time Factors
  • Tumor Necrosis Factor-alpha/blood

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